Wilhelm Camila Mörschbächer, Calsing Adriani Kunz, da Silva Luciano Basso
Curso de Biomedicina, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Environ Sci Pollut Res Int. 2015 Jun;22(11):8182-9. doi: 10.1007/s11356-014-3959-4. Epub 2014 Dec 17.
The aim of this study was to assess possible genotoxic effects on floriculturists in a region of the state of Rio Grande do Sul, in the south of Brazil, using the micronucleus test (MN) and comet assay. Thirty-seven floriculturists and 37 individuals not exposed to pesticides participated in the study. The micronucleus test was performed with epithelial cells of the oral mucosa. In the microscopic analysis, 2000 cells were evaluated per subject, verifying the frequency of MN and the frequency of other nuclear abnormalities (nuclear buds, binucleated cells, and karyorrhexis). For the comet assay in the peripheral blood lymphocytes, 100 cells were classified in five classes, according to the migration of DNA fragments, thereby generating the frequency of damaged cells and the damage index. There was no difference between the exposed and control groups in the frequencies of MN and other nuclear abnormalities in the epithelial cells of the oral mucosa. However, the comet assay showed that both the frequency of DNA damaged cells and the damage index were significantly greater in the exposed group. The results therefore indicate that floriculturists are exposed to mixtures of pesticides with genotoxic potential.
本研究的目的是利用微核试验（MN）和彗星试验，评估巴西南部南里奥格兰德州一个地区花卉种植者可能受到的遗传毒性影响。37名花卉种植者和37名未接触农药的个体参与了该研究。微核试验采用口腔黏膜上皮细胞进行。在显微镜分析中，每位受试者评估2000个细胞，核实微核频率以及其他核异常（核芽、双核细胞和核溶解）的频率。对于外周血淋巴细胞的彗星试验，根据DNA片段的迁移情况将100个细胞分为五类，从而得出受损细胞频率和损伤指数。口腔黏膜上皮细胞中微核及其他核异常的频率在暴露组和对照组之间没有差异。然而，彗星试验表明，暴露组中DNA受损细胞的频率和损伤指数均显著更高。因此，结果表明花卉种植者接触到了具有遗传毒性潜力的农药混合物。
