Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, 060-0810, Japan,
J Plant Res. 2015 Jan;128(1):17-25. doi: 10.1007/s10265-014-0692-5. Epub 2014 Dec 17.
Signaling molecules move between cells to form a characteristic distribution pattern within a developing organ; thereafter, they spatiotemporally regulate organ development. A key question in this process is how the signaling molecules robustly form the precise distribution on a tissue scale in a reproducible manner. Despite of an increasing number of quantitative studies regarding the mobility of signaling molecules, the detail mechanism of organogenesis via intercellular signaling is still unclear. We here review the potential advantages of plant development to address this question, focusing on the cytoplasmic continuity of plant cells through the plasmodesmata. The plant system would provide a unique opportunity to define the simple transportation mode of diffusion process, and, hence, the mechanism of organogenesis via intercellular signaling. Based on the advances in the understanding of intercellular signaling at the molecular level and in the quantitative imaging techniques, we discuss our current challenges in measuring the mobility of signaling molecules for deciphering plant organogenesis.
信号分子在细胞间移动，在发育器官内形成特征分布模式；此后，它们时空调节器官发育。这个过程中的一个关键问题是，信号分子如何以可重复的方式在组织尺度上稳健地形成精确的分布。尽管关于信号分子的流动性的定量研究越来越多，但通过细胞间信号传递进行器官发生的详细机制仍不清楚。在这里，我们回顾了植物发育的潜在优势，以解决这个问题，重点是质膜通过胞间连丝的细胞质连续性。植物系统将为定义扩散过程的简单运输模式提供一个独特的机会，从而为细胞间信号传递的器官发生机制提供一个独特的机会。基于对分子水平上细胞间信号传递和定量成像技术的理解的进展，我们讨论了我们目前在测量信号分子的流动性以破解植物器官发生方面的挑战。
