Fournier Gislene Fátima da Silva Rocha, Lopes Marcos Gomes, Marcili Arlei, Ramirez Diego Garcia, Acosta Igor Cunha Lima, Ferreira Juliana Isabel Giuli da Silva, Cabral Aline Diniz, Lima Júlia Tereza Ribeiro de, Pena Hilda Fátima de Jesus, Dias Ricardo Augusto, Gennari Solange Maria
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo - USP, São Paulo, SP, Brazil.
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo - USP, São Paulo, SP, Brazil.
Rev Bras Parasitol Vet. 2014 Oct-Dec;23(4):501-8. doi: 10.1590/S1984-29612014092. Epub 2014 Dec 1.
Toxoplasmosis stands out as a global disease that has felines as definitive hosts. In the municipality of Natal, Rio Grande do Norte State, Brazil, two parks are notable for their ecological and social importance. This study aimed to investigate the presence of Toxoplasma gondii in short hair cats, bats and small non-volant mammals in these two ecological reserves. Altogether, biological samples were obtained from 154 mammals, 92 wild animals from both areas and 62 domestic cats of the Parque da Cidade. In total, 22 (53.7%) non-volant wild mammals, 11 (21.5%) bats and 28 (52.8%) cats were positive for IgG anti-T. gondii antibodies using the Modified Agglutination Test (≥ 25). It was possible to detect the presence of T. gondii DNA, by means of a molecular amplification of a B1 gene fragment (155bp), in 92 tissue samples from wild animals, including Didelphis albiventris, Monodelphis domestica, Artibeus lituratus, Carollia perspicillata and Glossophaga soricina. Of the 62 cats examined by the same molecular method, T. gondii DNA could be detected in 4 cats. In this study, it was observed the circulation of T. gondii in wild species and domestic cats, demonstrating the involvement of wild and domestic animals in the cycle of T. gondii.
弓形虫病是一种以猫科动物为终宿主的全球性疾病。在巴西北里奥格兰德州纳塔尔市，有两个公园因其生态和社会重要性而备受关注。本研究旨在调查这两个生态保护区内短毛猫、蝙蝠和小型非飞行哺乳动物中弓形虫的存在情况。总共从154只哺乳动物身上采集了生物样本，其中包括来自两个区域的92只野生动物和城市公园的62只家猫。使用改良凝集试验（≥25）检测发现，共有22只（53.7%）非飞行野生哺乳动物、11只（21.5%）蝙蝠和28只（52.8%）猫的抗弓形虫IgG抗体呈阳性。通过对B1基因片段（155bp）进行分子扩增，在92份野生动物组织样本中检测到了弓形虫DNA，这些野生动物包括白腹袋鼹、家鼩负鼠、白喉卷尾猴、食果蝙蝠和长舌蝠。在通过相同分子方法检测的62只猫中，有4只检测到了弓形虫DNA。在本研究中，观察到弓形虫在野生物种和家猫中的传播，表明野生动物和家畜参与了弓形虫的传播循环。
