Griepenburg Julianne C, Sood Nimil, Vargo Kevin B, Williams Dewight, Rawson Jeff, Therien Michael J, Hammer Daniel A, Dmochowski Ivan J
Department of Chemistry, University of Pennsylvania , 231 South 34th Street, Philadelphia, Pennsylvania 19104, United States.
Langmuir. 2015 Jan 20;31(2):799-807. doi: 10.1021/la5036689. Epub 2015 Jan 7.
Polymersomes are bilayer vesicles that self-assemble from amphiphilic diblock copolymers, and provide an attractive system for the delivery of biological and nonbiological molecules due to their environmental compatibility, mechanical stability, synthetic tunability, large aqueous core, and hyperthick hydrophobic membrane. Herein, we report a nanoscale photoresponsive polymersome system featuring a meso-to-meso ethyne-bridged bis[(porphinato)zinc] (PZn2) fluorophore hydrophobic membrane solute and dextran in the aqueous core. Upon 488 nm irradiation in solution or in microinjected zebrafish embryos, the polymersomes underwent deformation, as monitored by a characteristic red-shifted PZn2 emission spectrum and confirmed by cryo-TEM. The versatility of this system was demonstrated through the encapsulation and photorelease of a fluorophore (FITC), as well as two different metal ions, Zn(2+) and Ca(2+).
聚合物囊泡是由两亲性二嵌段共聚物自组装形成的双层囊泡，由于其环境相容性、机械稳定性、合成可调性、较大的水相核心和超厚的疏水膜，为生物和非生物分子的递送提供了一个有吸引力的系统。在此，我们报道了一种纳米级光响应聚合物囊泡系统，其特征在于疏水膜溶质为中对中乙炔桥连双(卟吩)锌荧光团，水相核心中含有葡聚糖。在溶液中或显微注射到斑马鱼胚胎中后，用488nm光照射，聚合物囊泡发生变形，通过特征性的红移PZn2发射光谱监测，并通过冷冻透射电子显微镜证实。通过包裹和光释放一种荧光团(FITC)以及两种不同的金属离子Zn(2+)和Ca(2+)，证明了该系统的多功能性。
