Bezerra Daniel P, Ferreira Paulo Michel P, Machado Camila Maria L, de Aquino Nayara C, Silveira Edilberto R, Chammas Roger, Pessoa Claudia
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil.
Departamento de Biofísica e Fisiologia/Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Teresina, Piauí, Brazil.
Planta Med. 2015 Jan;81(1):15-9. doi: 10.1055/s-0034-1383363. Epub 2014 Dec 17.
Piper tuberculatum, popularly known in Brazil as "jaborandi falso" and "pimenta darta", is widely used in folk medicine for the treatment of several diseases. In this study, the in vivo hollow fiber assay was used to investigate the antitumour efficacy of the crude extract and piplartine obtained from P. tuberculatum roots. Human glioblastoma (SF-295) and colon carcinoma (HCT-8) cell lines were used. In vitro cytotoxicity was assayed by the MTT assay. In the hollow fiber assay, nude mice implanted with tumour cells in hollow fibers were treated for four consecutive days via the intraperitoneal route, and tumour cell populations were assessed by the MTT assay. Both the crude extract and piplartine displayed cytotoxicity. In the hollow fiber assay, tumour growth inhibition rates were 24.6-54.8 % for the crude extract and 33.7-62.2 % for piplartine. No signal of toxicity was noticed. In conclusion, the crude extract and piplartine obtained from P. tuberculatum roots displayed in vitro and in vivo anticancer efficacy.
胡椒（Piper tuberculatum）在巴西俗称“假毛果芸香”和“达尔塔胡椒”，在民间医学中广泛用于治疗多种疾病。在本研究中，采用体内中空纤维试验来研究从胡椒根中获得的粗提物和胡椒碱的抗肿瘤功效。使用了人胶质母细胞瘤（SF - 295）和结肠癌细胞系（HCT - 8）。通过MTT试验测定体外细胞毒性。在中空纤维试验中，将植入中空纤维内肿瘤细胞的裸鼠通过腹腔途径连续治疗四天，并通过MTT试验评估肿瘤细胞群体。粗提物和胡椒碱均显示出细胞毒性。在中空纤维试验中，粗提物的肿瘤生长抑制率为24.6 - 54.8%，胡椒碱为33.7 - 62.2%。未观察到毒性信号。总之，从胡椒根中获得的粗提物和胡椒碱在体外和体内均显示出抗癌功效。
