Han Wen Bo, Dou Huan, Yuan Wei Hua, Gong Wei, Hou Ya Yi, Ng Seik Weng, Tan Ren Xiang
Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, P. R. China.
Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia and Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Planta Med. 2015 Jan;81(2):145-51. doi: 10.1055/s-0034-1383392. Epub 2014 Dec 17.
The endophytic fungus Guignardia mangiferae isolated from Ilex cornuta leaves was shown to produce a family of meroterpenes with toll-like receptor 3 regulating activity (1-9), of which 1-3 possessed new structures. The absolute stereochemistry of 1-3 was assigned through a combination of nuclear magnetic resonance experiments, chemical derivation, CD spectra, and single-crystal X-ray diffraction analyses (CuK α ). The precursor labeled cultivation suggests that these meroterpenes are most likely assembled through terpenoid-shikimate pathways. Moreover, meroterpenes 1-3, 5-7, and 9 selectively upregulate, but 4 and 8 downregulate the toll-like receptor 3 expression in mouse dendritic cells at 10.0 µM.
从枸骨叶中分离出的内生真菌芒果球座菌被证明能产生一系列具有Toll样受体3调节活性的半萜（1-9），其中1-3具有新结构。通过核磁共振实验、化学衍生、圆二色光谱和单晶X射线衍射分析（CuKα）相结合的方法确定了1-3的绝对立体化学结构。前体标记培养表明，这些半萜很可能是通过萜类-莽草酸途径组装而成。此外，在10.0µM浓度下，半萜1-3、5-7和9能选择性地上调，而4和8则下调小鼠树突状细胞中Toll样受体3的表达。
