Okada H
Nihon Shokakibyo Gakkai Zasshi. 1989 May;86(5):1079-88.
Prolyl hydroxylase (PH) is an enzyme acting in early stage of collagen synthesis. We have emphasized the significance of the measurement of serum PH (SIRPH) in relation to liver fibrosis mainly in patients with alcoholic liver disease (ALD). In this study, we determined the localization and positivity of PH by tissue PH stain method (Avidin Biotin Complex method) to clarify the differences in fibrosis between ALD (25 cases) and non-alcoholic liver diseases (non-ALD, 47 cases). Tissue PH was found to be positive in liver cells around Glisson's sheath in early stage of fibrosis, and then in liver cells left within septa and also in mesenchymal cells in the sinusoidal wall as fibrosis progressed. Although there were basically no marked differences between ALD and non-ALD, ALD tended to show stronger tissue PH positivity for a degree of fibrosis, PH positivity in parenchymal cells was especially remarkable around pericellular fibrosis in ALD. These results clearly reflected the important role of liver parenchymal cells in progression of fibrosis.
脯氨酰羟化酶（PH）是一种在胶原蛋白合成早期起作用的酶。我们主要在酒精性肝病（ALD）患者中强调了血清PH（SIRPH）测量与肝纤维化的相关性。在本研究中，我们采用组织PH染色法（抗生物素蛋白-生物素复合物法）确定PH的定位和阳性情况，以阐明ALD（25例）和非酒精性肝病（非ALD，47例）之间纤维化的差异。在纤维化早期，组织PH在肝门管区周围的肝细胞中呈阳性，随着纤维化进展，在间隔内残留的肝细胞以及窦壁间充质细胞中也呈阳性。虽然ALD和非ALD之间基本没有明显差异，但在一定程度的纤维化情况下，ALD的组织PH阳性倾向更强，在ALD中，实质细胞中的PH阳性在细胞周围纤维化处尤为显著。这些结果清楚地反映了肝实质细胞在纤维化进展中的重要作用。
