一种用于直接监测病毒感染和中和抗体筛选的重组禽白血病病毒J亚群。

A recombinant avian leukosis virus subgroup j for directly monitoring viral infection and the selection of neutralizing antibodies.

作者信息

Wang Qi, Li Xiaofei, Ji Xiaolin, Wang Jingfei, Shen Nan, Gao Yulong, Qi Xiaole, Wang Yongqiang, Gao Honglei, Zhang Shide, Wang Xiaomei

机构信息

Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China.

Centre for Animal Infectious Disease Diagnosis and Technical Services and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China.

出版信息

PLoS One. 2014 Dec 18;9(12):e115422. doi: 10.1371/journal.pone.0115422. eCollection 2014.

Avian leukosis virus subgroup J (ALV-J) has induced serious clinical outbreaks and has become a serious infectious disease of chickens in China. We describe here the creation of a recombinant ALV-J tagged with the enhanced green fluorescent protein (named rHPRS-103EGFP). We successfully utilize the rHPRS-103EGFP to visualize viral infection and for development of a simplified serum-neutralization test.