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基于增强型绿色荧光蛋白标记的经典猪瘟病毒的简化血清中和试验。

Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus.

机构信息

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.

出版信息

J Clin Microbiol. 2013 Aug;51(8):2710-2. doi: 10.1128/JCM.00859-13. Epub 2013 May 22.

DOI:10.1128/JCM.00859-13
PMID:23698523
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3719638/
Abstract

The neutralization immunofluorescence test (NIFT), currently used for detecting neutralizing antibodies (NAbs) against classical swine fever virus (CSFV), is time-consuming. Here, a simplified neutralization test based on enhanced green fluorescent protein (EGFP)-tagged CSFV (EGFP-NT) was developed for direct detection of anti-CSFV NAbs without immunostaining. The relative sensitivity and specificity between EGFP-NT and NIFT or blocking enzyme-linked immunosorbent assay (ELISA) were both 100%. The NAb titers by EGFP-NT and the blocking rates by blocking ELISA showed a good correlation.

摘要

中和免疫荧光试验（NIFT）目前用于检测针对经典猪瘟病毒（CSFV）的中和抗体（NAbs），但较为耗时。本研究构建了基于增强型绿色荧光蛋白（EGFP）标记的 CSFV（EGFP-NT）的简化中和试验，可直接检测抗 CSFV NAbs，无需免疫染色。EGFP-NT 与 NIFT 或阻断酶联免疫吸附试验（ELISA）之间的相对灵敏度和特异性均为 100%。EGFP-NT 的 NAb 效价与阻断 ELISA 的阻断率具有良好的相关性。

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本文引用的文献

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Arch Virol. 2013 Apr;158(4):901-7. doi: 10.1007/s00705-012-1548-8. Epub 2012 Nov 29.
2
Comprehensive evaluation of the adenovirus/alphavirus-replicon chimeric vector-based vaccine rAdV-SFV-E2 against classical swine fever.基于腺病毒/甲病毒复制子嵌合载体的疫苗 rAdV-SFV-E2 对古典猪瘟的综合评估。
Vaccine. 2013 Jan 7;31(3):538-44. doi: 10.1016/j.vaccine.2012.11.013. Epub 2012 Nov 12.
3
Two newly developed E(rns)-based ELISAs allow the differentiation of Classical Swine Fever virus-infected from marker-vaccinated animals and the discrimination of pestivirus antibodies.两种新开发的基于 E(rns) 的 ELISA 允许区分经典猪瘟病毒感染动物和标记疫苗接种动物，并区分口蹄疫抗体。
Vet Microbiol. 2013 Jan 25;161(3-4):274-85. doi: 10.1016/j.vetmic.2012.07.046. Epub 2012 Aug 3.
4
Immunogenic and replicative properties of classical swine fever virus replicon particles modified to induce IFN-α/β and carry foreign genes.修饰后可诱导 IFN-α/β 并携带外源基因的经典猪瘟病毒复制子颗粒的免疫原性和复制特性。
Vaccine. 2011 Feb 4;29(7):1491-503. doi: 10.1016/j.vaccine.2010.12.026. Epub 2010 Dec 22.
5
Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus.表达经典猪瘟病毒 E2 蛋白的重组腺病毒的构建及效力评价。
Res Vet Sci. 2010 Feb;88(1):77-82. doi: 10.1016/j.rvsc.2009.06.005. Epub 2009 Jul 7.
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Baculovirus surface display of E2 envelope glycoprotein of classical swine fever virus and immunogenicity of the displayed proteins in a mouse model.猪瘟病毒E2包膜糖蛋白的杆状病毒表面展示及展示蛋白在小鼠模型中的免疫原性
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8
Interaction of classical swine fever virus with dendritic cells.经典猪瘟病毒与树突状细胞的相互作用。
J Gen Virol. 2004 Jun;85(Pt 6):1633-1641. doi: 10.1099/vir.0.19716-0.
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[Differential diagnosis of classical swine fever and border disease: seroepidemiological investigation of a pestivirus infection on a mixed sheep and swine farm].[经典猪瘟与边界病的鉴别诊断：一个羊猪混养农场瘟病毒感染的血清流行病学调查]
Dtsch Tierarztl Wochenschr. 2001 May;108(5):210-3.
10
A new assay for classical swine fever virus based on cytopathogenicity in porcine kidney cell line FS-L3.一种基于猪肾细胞系FS-L3细胞病变效应的经典猪瘟病毒新检测方法。
J Virol Methods. 1998 Jan;70(1):93-101. doi: 10.1016/s0166-0934(97)00175-4.