Brandenburg Boerries, Zhuang Xiaowei
Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA.
Nat Rev Microbiol. 2007 Mar;5(3):197-208. doi: 10.1038/nrmicro1615.
What could be a better way to study virus trafficking than 'miniaturizing oneself' and 'taking a ride with the virus particle' on its journey into the cell? Single-virus tracking in living cells potentially provides us with the means to visualize the virus journey. This approach allows us to follow the fate of individual virus particles and monitor dynamic interactions between viruses and cellular structures, revealing previously unobservable infection steps. The entry, trafficking and egress mechanisms of various animal viruses have been elucidated using this method. The combination of single-virus trafficking with systems approaches and state-of-the-art imaging technologies should prove exciting in the future.
还有什么比“将自己缩小”并“搭乘病毒颗粒”进入细胞的旅程更好的方式来研究病毒运输呢？在活细胞中进行单病毒追踪有可能为我们提供可视化病毒旅程的手段。这种方法使我们能够追踪单个病毒颗粒的命运，并监测病毒与细胞结构之间的动态相互作用，揭示以前无法观察到的感染步骤。利用这种方法已经阐明了各种动物病毒的进入、运输和释放机制。单病毒运输与系统方法和最先进的成像技术相结合，在未来应该会令人兴奋。
