Fortuna Claudia, Baggieri Melissa, Marchi Antonella, Benedetti Eleonora, Bucci Paola, Del Manso Martina, Declich Silvia, Iannazzo Stefania, Pompa Maria Grazia, Nicoletti Loredana, Magurano Fabio
Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Rome, Italy.
Ann Ist Super Sanita. 2014;50(4):341-4. doi: 10.4415/ANN_14_04_08.
The European Regional Office of the World Health Organization (WHO/Europe) developed a strategic approach to stop the indigenous transmission of measles in its 53 Member States by 2015. This study describes the measles laboratory surveillance activity performed by the National Reference Laboratory for Measles and Rubella at the Italian National Institute of Health (Istituto Superiore di Sanità) during 2010.
Urine, oral fluid and capillary blood samples from 211 suspected measles cases arrived to the NRL from different regions of Italy for confirmation of the clinical diagnosis. Serological and/or molecular assays were performed; after molecular detection, positive samples were sequenced and genotyped.
85% (180/211) of the specimens were confirmed as measles cases and 139 of these were analyzed phylogenetically. The phylogenetic analysis revealed a co-circulation of D4 and D8 genotypes for the reviewed period.
世界卫生组织欧洲区域办事处（WHO/欧洲）制定了一项战略方针，以在2015年前阻止麻疹在其53个成员国的本土传播。本研究描述了2010年期间意大利国家卫生研究院（Istituto Superiore di Sanità）的麻疹和风疹国家参考实验室开展的麻疹实验室监测活动。
来自意大利不同地区的211例疑似麻疹病例的尿液、口腔液和毛细血管血样本被送至国家参考实验室以确诊临床诊断。进行了血清学和/或分子检测；分子检测后，对阳性样本进行测序和基因分型。
85%（180/211）的标本被确诊为麻疹病例，其中139例进行了系统发育分析。系统发育分析显示，在审查期间D4和D8基因型共同流行。
