Sugiyama Tomoko, Uo Motohiro, Wada Takahiro, Omagari Daisuke, Komiyama Kazuo, Noguchi Tadahide, Jinbu Yoshinori, Kusama Mikio
Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.
Biometals. 2015 Feb;28(1):11-20. doi: 10.1007/s10534-014-9796-6. Epub 2014 Dec 19.
The effects of dissolved elements from metal dental restorations are a major concern in lesions of the oral mucosa, and the evaluation of accumulated metal elements, especially their distribution and chemical state, is essential for determining the precise effects of trace metals. In this study, X-ray fluorescence with synchrotron radiation (SR-XRF) and particle-induced X-ray emission (PIXE) were applied for distribution analysis of the trace metal elements contained in the oral mucosa, and the chemical states of the elements were estimated using X-ray absorption fine structure (XAFS) analysis. Appropriate combination of these analysis techniques, particularly SR-XRF and PIXE, to visualize the distributions of the elements in the oral mucosa allowed for the observation and evaluation of accumulated metal ions and debris. Importantly, the analyses in this study could be carried out using conventional histopathological specimens without damaging the specimens. Therefore, this method would be applicable for the detection of accumulated trace metal elements in biopsy specimens from the oral mucosa.
金属牙科修复体中溶解元素的影响是口腔黏膜病变的一个主要关注点，评估累积的金属元素，特别是它们的分布和化学状态，对于确定痕量金属的精确影响至关重要。在本研究中，同步辐射X射线荧光（SR-XRF）和粒子诱导X射线发射（PIXE）被用于口腔黏膜中痕量金属元素的分布分析，并且使用X射线吸收精细结构（XAFS）分析来估计元素的化学状态。这些分析技术的适当组合，特别是SR-XRF和PIXE，用于可视化口腔黏膜中元素的分布，从而能够观察和评估累积的金属离子和碎片。重要的是，本研究中的分析可以使用传统的组织病理学标本进行，而不会损坏标本。因此，该方法适用于检测口腔黏膜活检标本中累积的痕量金属元素。
