Golasik Magdalena, Wrobel Pawel, Olbert Magdalena, Nowak Barbara, Czyzycki Mateusz, Librowski Tadeusz, Lankosz Marek, Piekoszewski Wojciech
Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, Krakow, 30-060, Poland.
Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, al. Mickiewicza 30, Krakow, 30-059, Poland.
Biometals. 2016 Jun;29(3):487-94. doi: 10.1007/s10534-016-9930-8. Epub 2016 Apr 4.
Most studies have focused on the biodistribution of titanium(IV) oxide as nanoparticles or crystals in organism. But several reports suggested that titanium is released from implant in ionic form. Therefore, gaining insight into toxicokinetics of Ti ions will give valuable information, which may be useful when assessing the health risks of long-term exposure to titanium alloy implants in patients. A micro synchrotron radiation-induced X-ray fluorescence (µ-SRXRF) was utilized to investigate the titanium distribution in the liver, spleen and kidneys of rats following single intravenous or 30-days oral administration of metal (6 mg Ti/b.w.) in ionic form. Titanium was mainly retained in kidneys after both intravenous and oral dosing, and also its compartmentalization in this organ was observed. Titanium in the liver was non-uniformly distributed-metal accumulated in single aggregates, and some of them were also enriched in calcium. Correlation analysis showed that metal did not displace essential elements, and in liver titanium strongly correlated with calcium. Two-dimensional maps of Ti distribution show that the location of the element is characteristic for the route of administration and time of exposure. We demonstrated that µ-SRXRF can provide information on the distribution of titanium in internal structures of whole organs, which helps in enhancing our understanding of the mechanism of ionic titanium accumulation in the body. This is significant due to the popularity of titanium implants and the potential release of metal ions from them to the organism.
大多数研究都集中在二氧化钛作为纳米颗粒或晶体在生物体内的生物分布上。但有几份报告表明，钛会以离子形式从植入物中释放出来。因此，深入了解钛离子的毒代动力学将提供有价值的信息，这在评估患者长期接触钛合金植入物的健康风险时可能会有所帮助。利用微同步辐射诱导X射线荧光（µ-SRXRF）技术，研究了大鼠单次静脉注射或连续30天口服离子形式的金属（6毫克钛/体重）后，肝脏、脾脏和肾脏中的钛分布情况。静脉注射和口服给药后，钛主要保留在肾脏中，并且在该器官中观察到了其分区情况。肝脏中的钛分布不均匀——金属聚集在单个聚集体中，其中一些还富含钙。相关性分析表明，金属没有取代必需元素，并且在肝脏中钛与钙强烈相关。钛分布的二维图表明，该元素的位置对于给药途径和暴露时间具有特征性。我们证明了µ-SRXRF可以提供有关钛在整个器官内部结构中分布的信息，这有助于增强我们对离子钛在体内积累机制的理解。鉴于钛植入物的普及以及金属离子从它们向生物体的潜在释放，这一点具有重要意义。
