Zóka András, Barna Gábor, Somogyi Anikó, Műzes Györgyi, Oláh Ágnes, Al-Aissa Zahra, Hadarits Orsolya, Kiss Katalin, Firneisz Gábor
2nd Department of Medicine, Semmelweis University , Budapest , Hungary .
Autoimmunity. 2015;48(5):289-97. doi: 10.3109/08916934.2014.992518. Epub 2014 Dec 19.
Regulatory T-cells (Treg) have a crucial role in limiting physiologic autoreactivity. Foxp3 is a master regulator transcription factor of Treg differentiation and active Treg cells express high levels of IL-2 receptor α-chain (CD25). The aim of our study was to assess the key markers of Treg cell function in type 1 diabetic (T1DM) and control subjects by flow cytometry. The proportion of CD25(-/low) cells among CD4(+)Foxp3(+) Treg cells was higher in T1DM patients that might suggest a shifted proportion of the incomplete/reserve and the fully active (CD4(+)Foxp3(+)CD25(+)) Treg cell subpopulations in T1DM, similarly to other Th1-mediated autoimmune diseases. In addition to the decreased expression of CD25 and CTLA-4 in T1DM patients, a positive correlation was observed between the CD25 expression on CD4(+) and the CTLA-4 expression in CD8(-) T-lymphocytes both in the T1DM and in the healthy control group. Our results suggest an impaired balance of CD25(+) and CD25(-/low) Treg cells in T1DM which might reflect a decreased late phase peripheral Treg activation even in patients with a mean disease duration of more than a decade.
调节性T细胞（Treg）在限制生理性自身反应性方面发挥着关键作用。Foxp3是Treg分化的主要调节转录因子，活化的Treg细胞表达高水平的白细胞介素-2受体α链（CD25）。我们研究的目的是通过流式细胞术评估1型糖尿病（T1DM）患者和对照受试者中Treg细胞功能的关键标志物。T1DM患者中CD4(+)Foxp3(+) Treg细胞中CD25(-/低)细胞的比例更高，这可能表明T1DM中不完全/储备型和完全活化型（CD4(+)Foxp3(+)CD25(+)）Treg细胞亚群的比例发生了变化，类似于其他Th1介导的自身免疫性疾病。除了T1DM患者中CD25和CTLA-4表达降低外，在T1DM组和健康对照组中，CD4(+)细胞上的CD25表达与CD8(-) T淋巴细胞中的CTLA-4表达之间均观察到正相关。我们的结果表明，T1DM中CD25(+)和CD25(-/低) Treg细胞的平衡受损，这可能反映了即使在平均病程超过十年的患者中，晚期外周Treg活化也有所降低。
