Zhang Xufang, Jiang Hongwei, Gong Qimei, Fan Chen, Huang Yihua, Ling Junqi
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia.
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China.
Biochem Biophys Res Commun. 2014 Aug 8;450(4):1547-52. doi: 10.1016/j.bbrc.2014.07.027. Epub 2014 Jul 11.
High mobility group box 1 protein (HMGB1) is a chromatin protein which can be released extracellularly, eliciting a pro-inflammatory response and promoting tissue repair process. This study aimed to examine the expression and distribution of HMGB1 and its receptor RAGE in inflamed dental pulp tissues, and to assess its effects on proliferation, migration and cytoskeleton of cultured human dental pulp cells (DPCs). Our data demonstrated that cytoplasmic expression of HMGB1 was observed in inflamed pulp tissues, while HMGB1 expression was confined in the nuclei in healthy dental pulp. The mRNA expression of HMGB1 and RAGE were significantly increased in inflamed pulps. In in vitro cultured DPCs, expression of HMGB1 in both protein and mRNA level was up-regulated after treated with lipopolysaccharide (LPS). Exogenous HMGB1 enhanced DPCs migration in a dose-dependent manner and induced the reorganization of f-actin in DPCs. Our results suggests that HMGB1 are not only involved in the process of dental pulp inflammation, but also play an important role in the recruitment of dental pulp stem cells, promoting pulp repair and regeneration.
高迁移率族蛋白B1（HMGB1）是一种染色质蛋白，可释放到细胞外，引发促炎反应并促进组织修复过程。本研究旨在检测HMGB1及其受体RAGE在炎症牙髓组织中的表达和分布，并评估其对培养的人牙髓细胞（DPCs）增殖、迁移和细胞骨架的影响。我们的数据表明，在炎症牙髓组织中观察到HMGB1的细胞质表达，而在健康牙髓中HMGB1表达局限于细胞核。炎症牙髓中HMGB1和RAGE的mRNA表达显著增加。在体外培养的DPCs中，用脂多糖（LPS）处理后，HMGB1在蛋白质和mRNA水平的表达均上调。外源性HMGB1以剂量依赖的方式增强DPCs迁移，并诱导DPCs中丝状肌动蛋白的重组。我们的结果表明，HMGB1不仅参与牙髓炎症过程，而且在牙髓干细胞的募集、促进牙髓修复和再生中起重要作用。
