Nakai Madoka, Harrison Robert L, Uchida Haruaki, Ukuda Rie, Hikihara Shohei, Ishii Kazuo, Kunimi Yasuhisa
Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183-8509, Japan.
Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service (USDA-ARS), Beltsville Agricultural Research Center, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
J Gen Virol. 2015 Apr;96(Pt 4):904-914. doi: 10.1099/jgv.0.000023. Epub 2014 Dec 18.
A granulovirus (GV) producing occlusion bodies (OBs) with an unusual appearance was isolated from Adoxophyes spp. larvae in the field. Ultrastructural observations revealed that its OBs were significantly larger and cuboidal in shape, rather than the standard ovo-cylindrical shape typical of GVs. N-terminal amino acid sequence analysis of the OB matrix protein from this virus suggested that this new isolate was a variant of Adoxophyes orana granulovirus (AdorGV). Bioassays of this GV (termed AdorGV-M) and an English isolate of AdorGV (termed AdorGV-E) indicated that the two isolates were equally pathogenic against larvae of Adoxophyes honmai. However, AdorGV-M retained more infectivity towards larvae after irradiation with UV light than did AdorGV-E. Sequencing and analysis of the AdorGV-M genome revealed little sequence divergence between this isolate and AdorGV-E. Comparison of selected genes among the two AdorGV isolates and other Japanese AdorGV isolates revealed differences that may account for the unusual OB morphology of AdorGV-M.
从田间茶小卷叶蛾幼虫中分离出一种产生外观异常的包涵体（OBs）的颗粒体病毒（GV）。超微结构观察显示，其OBs明显更大且呈立方体形状，而非GV典型的标准卵圆形柱状形状。对该病毒OB基质蛋白的N端氨基酸序列分析表明，这种新分离株是茶小卷叶蛾颗粒体病毒（AdorGV）的一个变体。对这种GV（称为AdorGV-M）和AdorGV的一个英国分离株（称为AdorGV-E）进行的生物测定表明，这两种分离株对茶细蛾幼虫的致病性相同。然而，与AdorGV-E相比，AdorGV-M在紫外线照射后对幼虫仍保留更多的感染力。对AdorGV-M基因组的测序和分析表明，该分离株与AdorGV-E之间几乎没有序列差异。对这两种AdorGV分离株和其他日本AdorGV分离株中选定基因的比较揭示了可能导致AdorGV-M异常OB形态的差异。
