Ishaque M
Centre de Recherche en Microbiologie appliquée, Université du Québec, Laval-des-Rapides, Ville de Laval, Canada.
Res Microbiol. 1989 Feb;140(2):83-93. doi: 10.1016/0923-2508(89)90042-9.
Oxidation of palmitic acid by whole-cell suspensions of Mycobacterium leprae free from host tissues was investigated using manometric techniques. After a lag period of about 6-8 h, M. leprae suspensions catalysed an active oxidation of palmitic acid, and the oxidative process (oxygen uptake) was quite sensitive to rotenone, atabrine, amytal, antimycin A and cyanide. The spectrophotometric observations indicated that the M. leprae cytochrome system, under anaerobic conditions, was reduced in the presence of palmitic acid which was completely oxidized by oxygen. These data provide direct evidence that M. leprae cells are capable of oxidizing palmitic acid, and that oxidation is mediated by the electron transport system using oxygen as the terminal electron acceptor.
利用测压技术研究了不含宿主组织的麻风分枝杆菌全细胞悬液对棕榈酸的氧化作用。经过约6 - 8小时的延迟期后，麻风分枝杆菌悬液催化了棕榈酸的活性氧化，并且氧化过程（氧气摄取）对鱼藤酮、阿的平、戊巴比妥、抗霉素A和氰化物相当敏感。分光光度观察表明，在厌氧条件下，麻风分枝杆菌的细胞色素系统在棕榈酸存在时会被还原，而棕榈酸会被氧气完全氧化。这些数据提供了直接证据，证明麻风分枝杆菌细胞能够氧化棕榈酸，并且氧化是由以氧气作为末端电子受体的电子传递系统介导的。
