Hale M J, Liptz T R, Paterson A C
Department of Anatomical Pathology, School of Pathology, South African Institute for Medical Research, Johannesburg.
S Afr Med J. 1989 Oct 7;76(7):329-30.
Twenty specimens from patients who had undergone oesophagectomy for invasive squamous carcinoma of the oesophagus were examined for morphological evidence of human papillomavirus infection; it was found in 13 specimens. Nineteen specimens showed focal epithelial hyperplasia of the non-neoplastic mucosa. The material was also submitted to immunoperoxidase and modified Feulgen staining to detect viral antigen. Positive Feulgen staining was detected in the superficial layers of the squamous mucosa in 15 specimens, while immunoperoxidase was entirely negative. This demonstrates a possible association between human papillomavirus and oesophageal carcinoma and that the modified Feulgen method may be more sensitive than immunoperoxidase for the detection of viral antigen. Electron microscopy and molecular hybridisation would have to be used for confirmation.
对20例因食管浸润性鳞状细胞癌接受食管切除术患者的标本进行检查，以寻找人乳头瘤病毒感染的形态学证据；在13例标本中发现了该病毒。19例标本显示非肿瘤性黏膜存在局灶性上皮增生。对材料还进行了免疫过氧化物酶和改良福尔根染色以检测病毒抗原。15例标本的鳞状黏膜表层检测到福尔根染色阳性，而免疫过氧化物酶检测完全为阴性。这表明人乳头瘤病毒与食管癌之间可能存在关联，且改良福尔根方法在检测病毒抗原方面可能比免疫过氧化物酶更敏感。必须使用电子显微镜和分子杂交进行确认。
