Kok T C, Nooter K, Tjong-A-Hung S P, Smits H L, Ter Schegget J T
Department of Medical Oncology, Erasmus University Hospital Rotterdam, The Netherlands.
Eur J Cancer. 1997 Oct;33(11):1865-8. doi: 10.1016/s0959-8049(97)85984-9.
Controversial results regarding the presence and role of human papillomavirus in the development of oesophageal squamous cell carcinoma have been published. We used multiple broad-spectrum polymerase chain reactions to identify HPV DNA in oesophageal carcinomas from a low-incidence area. Paraffin embedded- and snap-frozen specimens from oesophageal cancer tissues of 63 patients were examined with a PCR technique with several primer pairs, capable of detecting most known HPV types. In none of the oesophagus cancer tissues could HPV DNA be detected. The role of HPV in this type of carcinoma in a low incidence area remains unclear.
关于人乳头瘤病毒在食管鳞状细胞癌发生中的存在情况及作用，已有一些存在争议的研究结果发表。我们采用多种广谱聚合酶链反应，来鉴定来自一个低发地区的食管癌组织中的人乳头瘤病毒DNA。运用聚合酶链反应技术及几对引物，对63例患者食管癌组织的石蜡包埋标本和速冻标本进行检测，这些引物能够检测出大多数已知的人乳头瘤病毒类型。在所有食管癌组织中均未检测到人乳头瘤病毒DNA。在低发地区，人乳头瘤病毒在这类癌症中的作用仍不明确。
