Arroyo-Lopez Celia, Manolaraki Foteini, Saratsis Anastasios, Saratsi Katerina, Stefanakis Alexandros, Skampardonis Vasileios, Voutzourakis Nikolaos, Hoste Hervé, Sotiraki Smaragda
Veterinary Research Institute - Hellenic Agricultural Organization Demeter, 57001 Thermi, Thessaloniki, Greece - UMR 1225 IHAP INRA/ENVT, École Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse Cedex, France.
UMR 1225 IHAP INRA/ENVT, École Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse Cedex, France.
Parasite. 2014;21:71. doi: 10.1051/parasite/2014074. Epub 2014 Dec 22.
The aim of the study was to compare the in vivo anthelmintic activity of sainfoin hay (Onobrychis viciifolia) and carob pod meal (Ceratonia siliqua) against gastrointestinal nematodes. Seven days before infection, 64 naive lambs were assigned to four different groups: Group S received sainfoin hay and group CAR was fed with carob pods. The remaining lambs received lucerne hay (Medicago sativa) and were assigned to positive (non-treated, NT) and negative (treated, T) control groups (treatment with albendazole). On day 0, lambs were artificially trickle infected for 6 weeks, with a mixture of infective larvae of Haemonchus contortus and Trichostrongylus colubriformis. Parasitological and pathophysiological parameters were measured repeatedly during the 2-month study. Compared to the NT group, decreases in egg excretion were observed in the CAR and S groups with significant differences only found for sainfoin (p < 0.05). At necropsy, group S showed decreases in the total worm numbers of both nematode species with significant differences for H. contortus. In contrast, no differences were noticed for the CAR group. Compared to the NT group, lower values for fecundity of female H. contortus were found in the S and CAR groups, however differences were non-significant. No differences in body weight gains were found between groups. Consistent results were found showing significantly higher packed cell volume (PCV) values in the T and S groups compared to NT and CAR groups. Overall, these results confirm a positive effect associated with the feeding of lambs with tanniniferous resources on host resilience (PCV values) and against gastrointestinal parasitic nematodes by affecting some biological traits of worm populations (e.g. eggs per gram of faeces and worm numbers). However, the anthelmintic effects differed between the two tannin-containing resources, which might be associated with the quantity and/or quality of secondary metabolites (condensed tannins and/or other polyphenols).
本研究的目的是比较红豆草干草（红豆草）和角豆荚粉（角豆树）对胃肠道线虫的体内驱虫活性。在感染前7天，将64只未感染过的羔羊分为四个不同的组：S组饲喂红豆草干草，CAR组饲喂角豆荚。其余羔羊饲喂苜蓿干草（紫花苜蓿），并分为阳性（未处理，NT）和阴性（处理，T）对照组（用阿苯达唑处理）。在第0天，羔羊人工逐滴感染6周，感染捻转血矛线虫和蛇形毛圆线虫的感染性幼虫混合物。在为期2个月的研究中反复测量寄生虫学和病理生理学参数。与NT组相比，CAR组和S组的虫卵排泄量均有所下降，仅红豆草组有显著差异（p < 0.05）。尸检时，S组两种线虫的总虫数均减少，捻转血矛线虫有显著差异。相比之下，CAR组未发现差异。与NT组相比，S组和CAR组雌性捻转血矛线虫的繁殖力较低，但差异不显著。各组之间体重增加无差异。结果一致表明，与NT组和CAR组相比，T组和S组的红细胞压积（PCV）值显著更高。总体而言，这些结果证实，用含单宁的资源喂养羔羊对宿主恢复力（PCV值）以及通过影响蠕虫种群的一些生物学特性（如每克粪便中的虫卵和虫数）对抗胃肠道寄生线虫具有积极作用。然而，两种含单宁资源的驱虫效果有所不同，这可能与次生代谢产物（缩合单宁和/或其他多酚）的数量和/或质量有关。
