Deng Qianchun, Yu Xiao, Xu Jiqu, Wang Lan, Huang Fenghong, Huang Qingde, Liu Changsheng, Ma Fangli
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 2 Xudong Second Road, Wuhan 430062, China.
Lipids Health Dis. 2014 Dec 19;13:198. doi: 10.1186/1476-511X-13-198.
Micronutrients in oil reduce one or more risk factors of cardiovascular diseases, while the contents of micronutrients in oil are relatively poor, which is insufficient to reverse the metabolic disorders at different stages of progress. The aim of this study was to investigate the effects of endogenous micronutrients in optimized cold-pressed rapeseed oil and restoratively added or fortified micronutrients in traditional refined rapeseed oil (restoring micronutrients to be nearly equal to or significantly higher than levels in crude rapeseed oil) on the antioxidant status and lipid profile in high-fat fed rats.
Male Wistar rats were fed high-fat diets containing different rapeseed oils for 4 weeks, including the standard refined rapeseed oil(SRO), optimized cold-pressed rapeseed oil(CRO) and the traditional refined rapeseed oil with restorative addition or fortification of micronutrients (LF, HF-SRO).
CRO exhibited significant increases in contents of tocopherols (+13%), phytosterols (+34%), polyphenols (+92%) and phospholipids (+725%) compared with SRO, as well as the total antioxidant capacities (+82-125%) (p<0.05). While the HF-SRO revealed improved antioxidant properties in vitro than the CRO, which was comparable to LF-SRO. Significant improved plasma antioxidant capacities and lipid peroxidation evaluated by T-AOC, GSH, tocopherols and MDA were found in rats fed HF-SRO when compared with CRO and LF-SRO (p<0.05). Furthermore, HF-SRO also decreased the plasma and hepatic TC levels compared to CRO and LF-SRO, accompanying higher fecal cholesterol excretion (p<0.05).
The standard refined rapeseed oil with fortification, not restorative addition of micronutrients was comparable to the optimized cold-pressed rapeseed oil in improving the antioxidant status and lipid profile of high-fat fed rats.
油脂中的微量营养素可降低心血管疾病的一种或多种风险因素，然而油脂中微量营养素的含量相对较低，不足以逆转疾病进展不同阶段的代谢紊乱。本研究旨在探讨优化冷榨菜籽油中的内源性微量营养素以及传统精炼菜籽油中恢复添加或强化的微量营养素（将微量营养素恢复至接近或显著高于粗菜籽油中的水平）对高脂喂养大鼠抗氧化状态和血脂谱的影响。
雄性Wistar大鼠喂食含不同菜籽油的高脂饮食4周，包括标准精炼菜籽油（SRO）、优化冷榨菜籽油（CRO）以及恢复添加或强化微量营养素的传统精炼菜籽油（LF，HF-SRO）。
与SRO相比，CRO的生育酚含量显著增加（+13%）、植物甾醇含量显著增加（+34%）、多酚含量显著增加（+92%）以及磷脂含量显著增加（+725%），总抗氧化能力也显著增加（+82 - 125%）（p<0.05）。虽然HF-SRO在体外显示出比CRO更好的抗氧化性能，与LF-SRO相当。与CRO和LF-SRO相比，喂食HF-SRO的大鼠通过T-AOC、GSH、生育酚和MDA评估的血浆抗氧化能力和脂质过氧化显著改善（p<0.05）。此外，与CRO和LF-SRO相比，HF-SRO还降低了血浆和肝脏TC水平，同时粪便胆固醇排泄增加（p<0.05）。
强化而非恢复添加微量营养素的标准精炼菜籽油在改善高脂喂养大鼠的抗氧化状态和血脂谱方面与优化冷榨菜籽油相当。
