Yoon Jaewoo, Kasai Hiroaki
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, Republic of Korea,
Antonie Van Leeuwenhoek. 2015 Mar;107(3):655-61. doi: 10.1007/s10482-014-0360-8. Epub 2014 Dec 21.
A Gram-negative, strictly aerobic, beige-pigmented, non-motile, rod-shaped strain designated H-MN17(T) was isolated from seawater in Japan. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flavobacteriaceae within the phylum Bacteroidetes and that it showed highest sequence similarity (97.5 %) to Wenyingzhuangia marina D1(T). The hybridization values for DNA-DNA relatedness between the strains of H-MN17(T) and W. marina D1(T) were lower than 70 %, which is accepted as the phylogenetic definition of a novel species. The DNA G+C content of strain H-MN17(T) was 31.8 mol%; MK-6 was the major menaquinone; and anteiso-C15:0, anteiso-C17:0 and anteiso-C19:0 were identified as the major (>10 %) cellular fatty acids. A complex polar lipid profile was present consisting of phosphatidylethanolamine, three unidentified lipids, three unidentified glycolipids and three unidentified aminolipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel species of the genus Wenyingzhuangia for which the name Wenyingzhuangia heitensis sp. nov. is proposed. The type strain of W. heitensis sp. nov. is H-MN17(T) (=KCTC 42245(T) = NBRC 110601(T)).
从日本海水中分离出一株革兰氏阴性、严格需氧、米色色素沉着、无运动性的杆状菌株，命名为H-MN17(T)。基于16S rRNA基因序列的系统发育分析表明，该新分离菌株隶属于拟杆菌门黄杆菌科，与海洋文营庄氏菌D1(T)的序列相似性最高（97.5%）。H-MN17(T)菌株与海洋文营庄氏菌D1(T)菌株之间的DNA-DNA杂交值低于70%，这是新物种系统发育定义所认可的。菌株H-MN17(T)的DNA G+C含量为31.8 mol%；MK-6是主要的甲基萘醌；anteiso-C15:0、anteiso-C17:0和anteiso-C19:0被鉴定为主要的（>10%）细胞脂肪酸。存在复杂的极性脂质谱，由磷脂酰乙醇胺、三种未鉴定的脂质、三种未鉴定的糖脂和三种未鉴定的氨基脂质组成。基于独特的系统发育位置以及基因型和表型特征的组合，该菌株被认为代表文营庄氏菌属的一个新物种，为此提出名为黑氏文营庄氏菌（Wenyingzhuangia heitensis sp. nov.）。黑氏文营庄氏菌新种的模式菌株为H-MN17(T)（=KCTC 42245(T)=NBRC 110601(T)）。
