Yoon Jaewoo, Oku Naoya, Kasai Hiroaki
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, Republic of Korea,
Antonie Van Leeuwenhoek. 2015 Jun;107(6):1607-13. doi: 10.1007/s10482-015-0456-9. Epub 2015 Apr 21.
A Gram-negative, strictly aerobic, beige-pigmented, non-motile, rod-shaped bacterial strain designated N5DB13-4(T) was isolated from the red alga Gracilaria vermiculophylla (Rhodophyta) collected at Sodegaura Beach, Chiba, Japan. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate is affiliated with the family Flavobacteriaceae within the phylum Bacteroidetes and that it showed highest sequence similarity (97.3 %) to Wenyingzhuangia heitensis H-MN17(T). The hybridization values for DNA-DNA relatedness between the strains N5DB13-4(T) and W. heitensis H-MN17(T) were 34.1 ± 3.5 %, which is below the threshold accepted for the phylogenetic definition of a novel prokaryotic species. The DNA G+C content of strain N5DB13-4(T) was determined to be 31.8 mol%; MK-6 was identified as the major menaquinone; and the presence of iso-C15:0, iso-C15:0 3-OH and iso-C17:0 3-OH as the major (>10 %) cellular fatty acids. A complex polar lipid profile was present consisting of phosphatidylethanolamine, two unidentified glycolipids and four unidentified lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel species of the genus Wenyingzhuangia for which the name Wenyingzhuangia gracilariae sp. nov. is proposed. The type strain of W. gracilariae sp. nov. is N5DB13-4(T) (=KCTC 42246 (T)=NBRC 110602(T)).
从日本千叶县铫子市海滩采集的红藻龙须菜（红藻门）中分离出一株革兰氏阴性、严格需氧、米色色素沉着、无运动性、杆状的细菌菌株，命名为N5DB13-4(T)。基于16S rRNA基因序列的系统发育分析表明，该新分离株属于拟杆菌门黄杆菌科，与黑氏文营庄氏菌H-MN17(T)的序列相似性最高（97.3%）。菌株N5DB13-4(T)与黑氏文营庄氏菌H-MN17(T)之间的DNA-DNA杂交值为34.1±3.5%，低于新原核生物物种系统发育定义所接受的阈值。菌株N5DB13-4(T)的DNA G+C含量测定为31.8 mol%；MK-6被鉴定为主要的甲基萘醌；主要（>10%）细胞脂肪酸为异-C15:0、异-C15:0 3-OH和异-C17:0 3-OH。存在由磷脂酰乙醇胺、两种未鉴定的糖脂和四种未鉴定的脂质组成的复杂极性脂质谱。根据独特的系统发育位置以及基因型和表型特征的组合，该菌株被认为代表文营庄氏菌属的一个新物种，为此提出新名称龙须菜文营庄氏菌（Wenyingzhuangia gracilariae sp. nov.）。龙须菜文营庄氏菌新物种的模式菌株为N5DB13-4(T)（=KCTC 42246(T)=NBRC 110602(T)）。
