Anfinsen Kristin P, Berghoff Nora, Priestnall Simon L, Suchodolski Jan S, Steiner Jörg M, Allenspach Karin
Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, AL9 7TA, England.
Current address: NMBU School of Veterinary Science, Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences (NMBU), Box 8146 Dep., N-0033, Oslo, Norway.
Acta Vet Scand. 2014 Dec 21;56(1):90. doi: 10.1186/s13028-014-0090-y.
This study sought to correlate faecal and urinary N-methylhistamine (NMH) concentrations with resting versus degranulated duodenal mast cell numbers in dogs with chronic enteropathies (CE), and investigate correlations between intestinal mast cell activation and clinical severity of disease as assessed by canine chronic enteropathy clinical activity index (CCECAI), and between urinary and faecal NMH concentrations, mast cell numbers, and histopathological scores. Twenty-eight dogs with CE were included. Duodenal biopsies were stained with haematoxylin and eosin (H&E), toluidine blue, and by immunohistochemical labelling for tryptase. Duodenal biopsies were assigned a histopathological severity score, and duodenal mast cell numbers were counted in five high-power fields after metachromatic and immunohistochemical staining. Faecal and urinary NMH concentrations were measured by gas chromatography-mass spectrometry.
There was no correlation between the CCECAI and faecal or urinary NMH concentrations, mast cell numbers, or histopathological score - or between faecal or urinary NMH concentration and mast cell numbers. Post hoc analysis revealed a statistically significant difference in toluidine blue positive mast cells between two treatment groups (exclusion diet with/without metronidazole versus immunosuppression (IS)), with higher numbers among dogs not requiring IS.
Faecal and urinary NMH concentrations and duodenal mast cell numbers were not useful indicators of severity of disease as assessed by the CCECAI or histological evaluation. The number of duodenal mast cells was higher in dogs that did not need IS, i.e. in dogs responding to an exclusion diet (with/without metronidazole), than in dogs requiring IS. Further studies comparing the role of mast cells in dogs with different forms of CE are needed.
本研究旨在探讨患有慢性肠病（CE）的犬类粪便和尿液中N-甲基组胺（NMH）浓度与静息状态和脱颗粒十二指肠肥大细胞数量之间的相关性，并研究肠道肥大细胞活化与犬慢性肠病临床活动指数（CCECAI）评估的疾病临床严重程度之间的相关性，以及尿液和粪便NMH浓度、肥大细胞数量和组织病理学评分之间的相关性。纳入了28只患有CE的犬。十二指肠活检标本用苏木精和伊红（H&E）、甲苯胺蓝染色，并通过类胰蛋白酶免疫组织化学标记。对十二指肠活检标本进行组织病理学严重程度评分，并在变色和免疫组织化学染色后在五个高倍视野中计数十二指肠肥大细胞数量。通过气相色谱-质谱法测量粪便和尿液中NMH的浓度。
CCECAI与粪便或尿液NMH浓度、肥大细胞数量或组织病理学评分之间没有相关性，粪便或尿液NMH浓度与肥大细胞数量之间也没有相关性。事后分析显示，两个治疗组（排除饮食加/不加甲硝唑与免疫抑制（IS））之间甲苯胺蓝阳性肥大细胞存在统计学上的显著差异，不需要IS的犬中数量更多。
粪便和尿液NMH浓度以及十二指肠肥大细胞数量并不是通过CCECAI或组织学评估所评估的疾病严重程度的有用指标。不需要IS的犬，即对排除饮食（加/不加甲硝唑）有反应的犬，十二指肠肥大细胞数量高于需要IS的犬。需要进一步研究比较肥大细胞在不同形式CE犬中的作用。
