Hanifeh Mohsen, Sankari Satu, Rajamäki Minna M, Syrjä Pernilla, Kilpinen Susanne, Suchodolski Jan S, Heilmann Romy M, Guadiano Phillip, Lidbury Jonathan, Steiner Jörg M, Spillmann Thomas
Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, PO Box 57, Viikintie 49, 00014, Helsinki, Finland.
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, 5166616471, Iran.
BMC Vet Res. 2018 Apr 4;14(1):125. doi: 10.1186/s12917-018-1441-0.
Intestinal mucosal S100A12 and myeloperoxidase (MPO) are inflammatory biomarkers in humans with inflammatory bowel disease (IBD). However, these biomarkers have not been studied in the intestinal mucosa of dogs with chronic enteropathies (CE), even though dogs with CE have increased S100A12 concentrations in feces and serum. This study investigated mucosal S100A12 concentrations and MPO activities in both dogs with CE and healthy Beagles. ELISA (S100A12 concentrations) and spectrophotometric methods (MPO activity) were used. The associations of both biomarkers with canine IBD activity index (CIBDAI), histopathologic findings, clinical outcome, and serum albumin concentrations were also investigated. We studied intestinal mucosal samples originating from different intestinal regions of 40 dogs with CE and 18 healthy Beagle dogs (duodenum, ileum, colon, and cecum).
Compared with healthy Beagles, mucosal S100A12 concentrations in dogs with CE were significantly higher in the duodenum (p < 0.0001) and colon (p = 0.0011), but not in the ileum (p = 0.2725) and cecum (p = 0.2194). Mucosal MPO activity of dogs with CE was significantly higher in the duodenum (p < 0.0001), ileum (p = 0.0083), colon (p < 0.0001), and cecum (p = 0.0474). Mucosal S100A12 concentrations in the duodenum were significantly higher if the inflammatory infiltrate consisted mainly of neutrophils (p = 0.0439) or macrophages (p = 0.037). Mucosal S100A12 concentrations also showed a significant association with the severity of total histopathological injury and epithelial injury in the colon (p < 0.05). Mucosal MPO activity showed a significant association (p < 0.05) with the severity of total histopathological injury, epithelial injury, and eosinophil infiltration in the duodenum. There was no significant association of both biomarkers with CIBDAI or clinical outcome.
This study showed that both mucosal S100A12 concentrations and MPO activities are significantly increased in the duodenum and colon of dogs with CE; mucosal MPO was also increased in the ileum and cecum. Future research should focus on assessing the clinical utility of S100A12 and MPO as diagnostic markers in dogs with CE.
肠道黏膜中的S100A12和髓过氧化物酶（MPO）是炎症性肠病（IBD）患者的炎症生物标志物。然而，尽管患有慢性肠病（CE）的犬类粪便和血清中的S100A12浓度升高，但这些生物标志物尚未在患有CE的犬类肠道黏膜中进行研究。本研究调查了患有CE的犬类和健康比格犬的黏膜S100A12浓度和MPO活性。采用酶联免疫吸附测定法（S100A12浓度）和分光光度法（MPO活性）。还研究了这两种生物标志物与犬类IBD活动指数（CIBDAI）、组织病理学结果、临床结局和血清白蛋白浓度之间的关联。我们研究了来自40只患有CE的犬类和18只健康比格犬不同肠道区域（十二指肠、回肠、结肠和盲肠）的肠道黏膜样本。
与健康比格犬相比，患有CE的犬类十二指肠（p < 0.0001）和结肠（p = 0.0011）中的黏膜S100A12浓度显著更高，但回肠（p = 0.2725）和盲肠（p = 0.2194）中则不然。患有CE的犬类十二指肠（p < 0.0001）、回肠（p = 0.0083）、结肠（p < 0.0001）和盲肠（p = 0.0474）中的黏膜MPO活性显著更高。如果炎症浸润主要由中性粒细胞（p = 0.0439）或巨噬细胞（p = 0.037）组成，十二指肠中的黏膜S100A12浓度显著更高。黏膜S100A12浓度还与结肠中总的组织病理学损伤和上皮损伤的严重程度显著相关（p < 0.05）。黏膜MPO活性与十二指肠中总的组织病理学损伤、上皮损伤和嗜酸性粒细胞浸润的严重程度显著相关（p < 0.05）。这两种生物标志物与CIBDAI或临床结局均无显著关联。
本研究表明，患有CE的犬类十二指肠和结肠中的黏膜S100A12浓度和MPO活性均显著升高；回肠和盲肠中的黏膜MPO也升高。未来的研究应侧重于评估S100A12和MPO作为患有CE的犬类诊断标志物的临床效用。
