Dong Hongxing, Zheng Meixia, Ou Yiling, Zhang Chunhong, Liu Lijia, Li Junqing, Yang Xiaoguang
Polymer Materials Research Center, Key Laboratory of Superlight Materials & Surface Technology, Ministry of Education, College of Material Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China.
Polymer Materials Research Center, Key Laboratory of Superlight Materials & Surface Technology, Ministry of Education, College of Material Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China.
J Chromatogr A. 2015 Jan 9;1376:172-6. doi: 10.1016/j.chroma.2014.12.009. Epub 2014 Dec 12.
This report describes novel pathway for the development of a new type of CSP. A chiral stationary phase (CSP) prepared by coating a molecularly imprinted polymer (MIP) on the surface of silica gel (SMIP-CSP) showed a high separation factor (3.39) in separating racemic 1,1'-binaphthalene-2,2'-diamine (DABN) by high performance liquid chromatography (HPLC). Being the crosslinking agent, ethylene glycol dimethacrylate (EGDMA) was copolymerized with the monomer, methacrylic acid (MAA), in the presence of (R)-DABN as the template molecules on the surface of the silica gel particles to produce the SMIP-CSPs. The effects of the pretreatment temperature, the (R)-DABN content and the type of silica gel and monomer in the SMIP-CSPs on the separation of the racemic DABN were systematically investigated.
本报告描述了一种新型手性固定相（CSP）的开发新途径。通过在硅胶表面涂覆分子印迹聚合物（MIP）制备的手性固定相（SMIP-CSP），在通过高效液相色谱（HPLC）分离外消旋1,1'-联萘-2,2'-二胺（DABN）时显示出高分离因子（3.39）。以乙二醇二甲基丙烯酸酯（EGDMA）作为交联剂，在硅胶颗粒表面以（R）-DABN为模板分子，使其与单体甲基丙烯酸（MAA）共聚，制备出SMIP-CSP。系统研究了SMIP-CSP中预处理温度、（R）-DABN含量以及硅胶和单体类型对外消旋DABN分离的影响。
