Granato Daniel, Margraf Tiago, Brotzakis Ioannis, Capuano Edoardo, van Ruth Saskia M
RIKILT - Inst. of Food Safety, Wageningen Univ. and Research Centre, P.O. Box 230, 6700, AE, Wageningen, The Netherlands; Food Quality and Design Group, Wageningen Univ. and Research Centre, P.O. Box 17, 6700, AA, Wageningen, The Netherlands; Department of Food Engineering, State Univ. of Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, Brazil.
J Food Sci. 2015 Jan;80(1):C55-65. doi: 10.1111/1750-3841.12722. Epub 2014 Dec 19.
The objectives of this study were to characterize organic, biodynamic, and conventional purple grape juices (n = 31) produced in Europe based on instrumental taste profile, antioxidant activity, and some chemical markers and to propose a multivariate statistical model to analyze their quality and try to classify the samples from the 3 different crop systems. Results were subjected to ANOVA, correlation, and regression analysis, principal component analysis (PCA), hierarchical cluster analysis (HCA), soft independent modeling of class analogy (SIMCA), and partial least-squares discriminant analysis (PLSDA). No statistical significant differences (P > 0.05) were observed among juices from the 3 crop systems. Using PCA and HCA, no clear separation among crop systems was observed, corroborating the ANOVA data. However, PCA showed that the producing region highly affects the chemical composition, electronic tongue parameters, and bioactivity of grape juices. In this sense, when organic and biodynamic were grouped as "nonconventional" juices, SIMCA model was able to discriminate 12 out of 13 organic/biodynamic juices and 17 out of 18 conventional juices, presenting an efficiency of 93.5%, while 11 out of 13 non-conventional and 100% conventional grape juices were correctly classified using PLSDA. The use of electronic tongue and the determination of antioxidant properties and major phenolic compounds have shown to be a quick and accurate analytical approach to assess the quality of grape juices.
本研究的目的是基于仪器味觉特征、抗氧化活性和一些化学指标,对欧洲生产的有机、生物动力和传统紫葡萄汁(n = 31)进行表征,并提出一种多元统计模型来分析其质量,并尝试对来自3种不同种植系统的样品进行分类。对结果进行了方差分析、相关性和回归分析、主成分分析(PCA)、层次聚类分析(HCA)、类类比软独立建模(SIMCA)和偏最小二乘判别分析(PLSDA)。在这3种种植系统的果汁中未观察到统计学上的显著差异(P > 0.05)。使用PCA和HCA,未观察到种植系统之间有明显的区分,这与方差分析数据一致。然而,PCA表明,产地对葡萄汁的化学成分、电子舌参数和生物活性有很大影响。从这个意义上说,当将有机和生物动力果汁归为“非传统”果汁时,SIMCA模型能够区分13种有机/生物动力果汁中的12种和18种传统果汁中的17种,效率为93.5%,而使用PLSDA正确分类了13种非传统果汁中的11种和100%的传统葡萄汁。使用电子舌以及测定抗氧化性能和主要酚类化合物已被证明是评估葡萄汁质量的一种快速准确的分析方法。