Sarioglu Nurhan, Bilen Cigdem, Cevik Celalettin, Gencer Nahit
Department of Pulmonary Diseases, Balikesir University School of Medicine, Balikesir, Turkey.
Department of Chemistry, Yildiz Technical University, Faculty of Arts and Science, Istanbul, Turkey.
Eurasian J Med. 2020 Jun;52(2):161-165. doi: 10.5152/eurasianjmed.2019.19122. Epub 2020 Jun 9.
Paraoxonase 1 (PON1) and arylesterase (ARE) enzymes have an important role in the prevention of oxidative stress which is related to the pathogenesis of chronic obstructive pulmonary disease (COPD). PON1 levels vary widely among individuals and ethnic groups, which is in part associated with polymorphisms.
We investigated PON1 and ARE activity and phenotype distribution in COPD patients and healthy individuals. Sixty six COPD patients and 59 control subjects were involved in the study. Serum PON1 and ARE activities were detected by spectrophotometric method. The ratio of salt-induced PON1 to ARE activity was used to determine phenotypes as QQ, QR, and RR.
COPD patients exhibited higher PON1 activity (199.1 vs 129.2, p=0.002) but lower ARE activity compared to control (21.3 vs 33.5, p=0.021). There was a significant difference between COPD and control group with respect to PON1 phenotype characteristics. RR phenotypic distribution was more common in the COPD group than in control (60.6% [95% CI: 48.8 - 72.3] versus 22.0 % [95% CI: 12.0 - 31.9], p=0.001). We also found that smoking (95.0% CI: 0.001-0.036, p<0.001) and RR phenotype (95.0% CI: 0.006 - 0.59, p=0.016) are independent determinants in COPD.
We found that RR phenotype was more common in COPD patients compared to control. Smoking and RR phenotype may be defined as independent factors associated with COPD.
对氧磷酶1（PON1）和芳基酯酶（ARE）在预防与慢性阻塞性肺疾病（COPD）发病机制相关的氧化应激中起重要作用。PON1水平在个体和种族群体中差异很大，这部分与基因多态性有关。
我们调查了COPD患者和健康个体的PON1和ARE活性及表型分布。66例COPD患者和59例对照受试者参与了本研究。采用分光光度法检测血清PON1和ARE活性。盐诱导的PON1与ARE活性之比用于确定QQ、QR和RR表型。
与对照组相比，COPD患者的PON1活性较高（199.1对129.2，p = 0.002），但ARE活性较低（21.3对33.5，p = 0.021）。COPD组和对照组在PON1表型特征方面存在显著差异。RR表型分布在COPD组比对照组更常见（60.6% [95% CI：48.8 - 72.3] 对22.0% [95% CI：12.0 - 31.9]，p = 0.001）。我们还发现吸烟（95.0% CI：0.001 - 0.036，p < 0.001）和RR表型（95.0% CI：0.006 - 0.59，p = 0.016）是COPD的独立决定因素。
我们发现与对照组相比，RR表型在COPD患者中更常见。吸烟和RR表型可能被定义为与COPD相关的独立因素。
