Yang Meili, Chen Peihong, Jin Hua, Xie Xinmiao, Gao Ting, Yang Lili, Yu Xuemei
Department of endocrinology and Metabolism, Fengxian Central Hospital, Shanghai, China ; Department of endocrinology and Metabolism, The First Clinical Medical College of Suzhou University, Suzhou, China.
Department of endocrinology and Metabolism, Fengxian Central Hospital, Shanghai, China.
Diabetol Metab Syndr. 2014 Dec 5;6(1):133. doi: 10.1186/1758-5996-6-133. eCollection 2014.
Irisin is a novel myokine secreted in response to peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) activation through exercise. The first-degree relatives (FDRs) of type 2 diabetes mellitus (T2DM) patients bear a lifetime risk for developing T2DM, especially after 40 years old. However, the circulating irisin levels in middle-aged FDRs of T2DM is unclear. We therefore investigated the association between circulating irisin and pancreatic β-cell function in normal-glucose-tolerance (NGT) subjects.
In this cross-sectional study, we recruited 412 supposed healthy subjects aged 40-60 who were FDRs of T2DM patients but without previous diagnosis of T2DM. Of the 412 individuals, 254 had NGT and 60 were newly diagnosed T2DM based on the results of a 75 g oral glucose tolerance test (OGTT- World Health Organization diagnostic criteria). We measured irisin in the newly diagnosed T2DM group (n = 60) and in an age- and sex-matched NGT subgroups (n = 62). Serum irisin was quantified by ELISA, and its association with metabolic parameters was analysed by Pearson's correlation and multiple linear regression analyses.
There was no significant difference in serum irisin between middle-aged newly diagnosed T2DM patients and the NGT control group. Circulating irisin was correlated with haemoglobin A1c (r = 0.202, p = 0.026) and estimated glomerular filtration rate (r = 0.239, p = 0.010). Multiple linear regression revealed that only homeostasis model assessment-β (HOMA-β) was associated with irisin in NGT subjects after adjusting for confounding factors. However, similar analysis in T2DM did not reveal a significant association between circulating irisin and metabolic parameters.
There was no significant difference in serum irisin between middle-aged newly diagnosed T2DM patients and the NGT controls. Serum irisin level was closely related to HOMA-β in NGT, suggesting that irisin may play a crucial role in pancreatic β-cell function.
鸢尾素是一种新型的肌动蛋白，通过运动激活过氧化物酶体增殖物激活受体γ共激活因子-1α（PGC-1α）后分泌。2型糖尿病（T2DM）患者的一级亲属（FDRs）有患T2DM的终生风险，尤其是40岁以后。然而，T2DM中年FDRs的循环鸢尾素水平尚不清楚。因此，我们研究了正常糖耐量（NGT）受试者循环鸢尾素与胰腺β细胞功能之间的关系。
在这项横断面研究中，我们招募了412名年龄在40 - 60岁之间、为T2DM患者的FDRs但既往未诊断为T2DM的假定健康受试者。在这412名个体中，根据75克口服葡萄糖耐量试验（OGTT - 世界卫生组织诊断标准）的结果，254人有正常糖耐量，60人新诊断为T2DM。我们在新诊断的T2DM组（n = 60）和年龄及性别匹配的NGT亚组（n = 62）中测量了鸢尾素。通过酶联免疫吸附测定（ELISA）对血清鸢尾素进行定量，并通过Pearson相关性分析和多元线性回归分析其与代谢参数的关系。
新诊断的中年T2DM患者与NGT对照组之间血清鸢尾素无显著差异。循环鸢尾素与糖化血红蛋白（r = 0.202，p = 0.026）和估计肾小球滤过率（r = 0.239，p = 0.010）相关。多元线性回归显示，在调整混杂因素后，仅稳态模型评估-β（HOMA-β）与NGT受试者的鸢尾素相关。然而，在T2DM中进行的类似分析未发现循环鸢尾素与代谢参数之间存在显著关联。
新诊断的中年T2DM患者与NGT对照组之间血清鸢尾素无显著差异。血清鸢尾素水平与NGT中的HOMA-β密切相关，表明鸢尾素可能在胰腺β细胞功能中起关键作用。
