Wang Yanan, Kar Archana, Paterson Andrew, Kourentzi Katerina, Le Han, Ruchhoeft Paul, Willson Richard, Bao Jiming
Department of Electrical and Computer Engineering and Department of Chemical and Biomolecular Engineering, University of Houston , Houston, Texas 77204, United States.
Department of Electrical and Computer Engineering and Department of Chemical and Biomolecular Engineering, University of Houston , Houston, Texas 77204, United States ; Centro de Biotecnología FEMSA, Departamento de Biotecnología e Ingeniería de Alimentos, Tecnológico de Monterrey , Monterrey, NL 64849, Mexico.
ACS Photonics. 2014 Mar 19;1(3):241-245. doi: 10.1021/ph400111u. Epub 2014 Feb 6.
A high-throughput optical biosensing technique is proposed and demonstrated. This hybrid technique combines optical transmission of nanoholes with colorimetric silver staining. The size and spacing of the nanoholes are chosen so that individual nanoholes can be independently resolved in massive parallel using an ordinary transmission optical microscope, and, in place of determining a spectral shift, the brightness of each nanohole is recorded to greatly simplify the readout. Each nanohole then acts as an independent sensor, and the blocking of nanohole optical transmission by enzymatic silver staining defines the specific detection of a biological agent. Nearly 10000 nanoholes can be simultaneously monitored under the field of view of a typical microscope. As an initial proof of concept, biotinylated lysozyme (biotin-HEL) was used as a model analyte, giving a detection limit as low as 0.1 ng/mL.
一种高通量光学生物传感技术被提出并得到验证。这种混合技术将纳米孔的光传输与比色银染色相结合。纳米孔的尺寸和间距经过精心选择，以便使用普通透射光学显微镜在大规模并行情况下能够独立分辨各个纳米孔，并且，不是确定光谱位移，而是记录每个纳米孔的亮度，从而极大地简化了读数过程。然后，每个纳米孔都充当一个独立的传感器，酶促银染色对纳米孔光传输的阻断定义了对生物制剂的特异性检测。在典型显微镜的视野下可以同时监测近10000个纳米孔。作为概念的初步验证，生物素化溶菌酶（生物素-HEL）被用作模型分析物，检测限低至0.1 ng/mL。
