Christie Michelle P, Toth Istvan, Simerská Pavla
School of Chemistry & Molecular Biosciences, The University of Queensland, St. Lucia, QLD 4072, Australia.
Future Med Chem. 2014;6(18):2113-29. doi: 10.4155/fmc.14.130.
Lectin-glycan interactions play a role in biological processes, host-pathogen interactions and in disease. A more detailed understanding of these interactions is not only useful for the elucidation of their biological function but can also be applied in immunology, drug development and delivery and diagnostics. We review some commonly used biophysical techniques for studying lectin-glycan interactions; namely: frontal affinity chromatography, glycan/lectin microarray, surface plasmon resonance, electrochemical impedance spectroscopy, isothermal titration calorimetry, fluorescent assays, enzyme linked lectin sorbent assay and saturation transfer difference nuclear magnetic resonance spectroscopy. Each method is evaluated on efficiency, cost and throughput. We also consider the advantages and limitations of each technique and provide examples of their application in biology, drug discovery and delivery, immunology, glycoprofiling and biosensing.
凝集素-聚糖相互作用在生物过程、宿主-病原体相互作用及疾病中发挥作用。更详细地了解这些相互作用不仅有助于阐明其生物学功能，还可应用于免疫学、药物研发与递送以及诊断学。我们综述了一些用于研究凝集素-聚糖相互作用的常用生物物理技术，即：前沿亲和色谱法、聚糖/凝集素微阵列、表面等离子体共振、电化学阻抗谱、等温滴定量热法、荧光分析法、酶联凝集素吸附测定法和饱和转移差核磁共振波谱法。对每种方法的效率、成本和通量进行了评估。我们还考虑了每种技术的优缺点，并提供了它们在生物学、药物发现与递送、免疫学、糖谱分析和生物传感中的应用实例。
