Glycomics Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang-Gung University, Kwei-san, Tao-yuan, 33332, Taiwan.
Ta-Cheng Stem Cell Therapy Center, National Taiwan University, Taipei, Taiwan.
Glycoconj J. 2019 Apr;36(2):175-183. doi: 10.1007/s10719-019-09865-3. Epub 2019 Apr 16.
Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins were updated and upgraded. (ii) Based on the cross-specificities of applied lectins, a new classification system was introduced. (iii) Applications of lectins for the detection and identification of N-glycan and/or Tn glycotope in glycoconjugates were intergraded. (iv) The polyvalency of the glycotopes in glycans was found to play a critical role in glycan-lectin recognition. This is an unexplored area of glycobiology and one of the most promising directions toward the coming glycoscience transformation.
凝集素与我们已建立的酶联凝集素结合分析（ELLSA）和抑制研究相结合，已被广泛应用于多种糖缀合物识别研究中。在这篇简短的综述中，我们强调了以下几点：（i）Gal/GalNAc 特异性凝集素的识别特性得到了更新和升级。（ii）基于应用凝集素的交叉特异性，引入了一种新的分类系统。（iii）整合了凝集素在糖缀合物中 N-聚糖和/或 Tn 糖基识别中的应用。（iv）聚糖中糖基的多价性在糖-凝集素识别中起着关键作用。这是糖生物学中一个尚未探索的领域，也是未来糖科学转型最有前途的方向之一。
