Kostopoulos Ioannis V, Paterakis Georgios, Papadimitriou Konstantinos, Pavlidis Dimitrios, Tsitsilonis Ourania E, Papadhimitriou Stefanos I
Haematology Laboratory, "G. Gennimatas" Athens Regional General Hospital, Athens, Greece; Department of Animal and Human Physiology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece.
Genes Chromosomes Cancer. 2015 Apr;54(4):210-21. doi: 10.1002/gcc.22234. Epub 2014 Dec 23.
Monoclonal B-cell lymphocytosis (MBL) is the presence of small B-cell clones in the peripheral blood of healthy subjects. Most MBL have the characteristic phenotype of chronic lymphocyte leukemia (chronic lymphocytic leukemia (CLL)-like MBL), and depending on the number of monoclonal B-cells, may characterize a preclinical stage of the CLL. However, there are also MBL with an atypical (CD5(+) CD20(+/bright) CD23(dim/-) ) or a CD5(neg) phenotype, which remain largely unexplored. We performed an extended immunophenotypic, cytogenetic, and hematologic analysis in 75 CLL-like, 39 atypical, 50 CD5(neg) , and 7 biphenotypic MBL cases to detect differences or similarities among the MBL subsets. The phenotypic analysis showed expression variations in many surface markers and a wide spectrum of disease-specific phenotypes within each MBL subtype. Interphase fluorescent in situ hybridization analysis showed a different panel of aberrations according to the phenotype. Overall, del(13q14) and +12 were the most common abnormalities (39%), whereas del(11q13), del(17p13), and del(6q23) were detected only in 3, 1, and 0 cases, respectively. A comparison of MBL with overt chronic lymphoproliferations revealed common aspects in the preclinical state, regarding both the kind of cytogenetic aberrations detected and the lymphocyte composition. Our findings highlight not only the heterogeneity among MBL subsets but also indicate common biologic features which differentiate MBL from clinical disease.
单克隆B淋巴细胞增多症（MBL）是指健康受试者外周血中存在小B细胞克隆。大多数MBL具有慢性淋巴细胞白血病的特征性表型（慢性淋巴细胞白血病样MBL），根据单克隆B细胞的数量，可能代表慢性淋巴细胞白血病的临床前期阶段。然而，也存在具有非典型（CD5(+) CD20(+/明亮) CD23(暗淡/阴性)）或CD5(阴性)表型的MBL，对此研究尚少。我们对75例慢性淋巴细胞白血病样、39例非典型、50例CD5(阴性)和7例双表型MBL病例进行了扩展的免疫表型、细胞遗传学和血液学分析，以检测MBL各亚组之间的差异或相似性。表型分析显示，许多表面标志物存在表达差异，且每个MBL亚型内存在广泛的疾病特异性表型。间期荧光原位杂交分析显示，根据表型不同，畸变情况各异。总体而言，del(13q14)和+12是最常见的异常（39%），而del(11q13)、del(17p13)和del(6q23)分别仅在3例、1例和0例中检测到。将MBL与明显的慢性淋巴细胞增殖性疾病进行比较发现，在临床前期状态下，无论是检测到的细胞遗传学畸变类型还是淋巴细胞组成方面，都存在共同之处。我们的研究结果不仅突出了MBL各亚组之间的异质性，还表明了将MBL与临床疾病区分开来的共同生物学特征。
