Graillot Benoît, Berling Marie, Blachere-López Christine, Siegwart Myriam, Besse Samantha, López-Ferber Miguel
LGEI, Ecole des Mines d'Alès, Institut Mines-Telecom et Université de Montpellier Sud de France, 6, Avenue de Clavières, 30319 Alès, France.
INRA, 6, Avenue de Clavières, 30319 Alès, France.
Viruses. 2014 Dec 22;6(12):5135-44. doi: 10.3390/v6125135.
The NPP-R1 isolate of CpGV is able to replicate on CpGV-M-resistant codling moths. However, its efficacy is not sufficient to provide acceptable levels of control in natural (orchard) conditions. A laboratory colony derived from resistant codling moths was established, which exhibited a homogeneous genetic background and a resistance level more than 7000 fold. By successive cycles of replication of NPP-R1 in this colony, we observed a progressive increase in efficacy. After 16 cycles (isolate 2016-r16), the efficacy of the virus isolate was equivalent to that of CpGV-M on susceptible insects. This isolate was able to control both CpGV-M-susceptible and CpGV-M-resistant insects with similar efficacy. No reduction in the levels of occlusion body production in susceptible larvae was observed for 2016-r16 compared to CpGV-M.
苹果蠹蛾颗粒体病毒（CpGV）的NPP-R1分离株能够在对CpGV-M具有抗性的苹果蠹蛾上复制。然而，其防治效果不足以在自然（果园）条件下提供可接受的防治水平。建立了一个源自抗性苹果蠹蛾的实验室种群，该种群具有均匀的遗传背景且抗性水平超过7000倍。通过在该种群中对NPP-R1进行连续的复制循环，我们观察到其防治效果逐渐提高。经过16个循环（分离株2016-r16）后，该病毒分离株对敏感昆虫的防治效果与CpGV-M相当。该分离株能够以相似的防治效果控制对CpGV-M敏感和具有抗性的昆虫。与CpGV-M相比，未观察到2016-r16在敏感幼虫中产生的包涵体水平有所降低。
