[新生儿外周血白细胞中的沙眼衣原体DNA]

[Chlamydia trachomaatis DNA in leukocytes of peripheral blood from neonates].

作者信息

López-Hurtado Marcela, Cuevas-Recillas Karla N, Flores-Salazar Verónica R, Guerra-Infante Fernando M

机构信息

Laboratorio de Virología, Instituto Nacional de Perinatología, México D.F., México; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, México D.F., México.

出版信息

Enferm Infecc Microbiol Clin. 2015 Aug-Sep;33(7):458-63. doi: 10.1016/j.eimc.2014.09.019. Epub 2014 Dec 20.

DOI:10.1016/j.eimc.2014.09.019

PMID:25533744

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7103315/

Abstract

INTRODUCTION

Diagnosis of Chlamydia trachomatis infection in newborns is difficult; however, this diagnosis is performed by cell culture or by detection of IgM antibodies against C. trachomatis. Detection of C. trachomatis DNA in peripheral blood leukocytes using polymer chain reaction (PCR) may be a better tool for the diagnosis of infection by this pathogen.

MATERIAL AND METHODS

A total of 44 premature newborns, all weighing less than 2500g, were included in the study. A blood sample and nasopharyngeal lavages were obtained from each newborn. Leukocyte DNA was obtained by phenol-chloroform extraction technique. Detection of C. trachomatis was performed by amplifying the ompA gene using the PCR endpoint. Cell culture tests and the detection of IgM antibodies against C. trachomatis by microimmunofluorescence assay were also performed.

RESULTS

Twenty newborns were PCR-positive (45.5%), with this test being significantly associated with the presence of pneumonia (RR=2.28; 95%CI: 1.01 to 5.17; P=.035). The cell culture of nasopharyngeal lavage was positive in only 7 samples and no significant association was observed with any clinical or laboratory data. The titer of IgM antibodies against C. trachomatis associated with PCR-positive was 1:32 (RR=2.74; 95%CI: 1.21 to 6.23; P=.008), however this titer was not associated with the presence of pneumonia.

CONCLUSION

DNA detection in peripheral blood leukocytes could be useful for diagnosis of C. trachomatis infection.

摘要

引言

新生儿沙眼衣原体感染的诊断较为困难；然而，这种诊断是通过细胞培养或检测抗沙眼衣原体的IgM抗体来进行的。使用聚合酶链反应（PCR）检测外周血白细胞中的沙眼衣原体DNA可能是诊断这种病原体感染的更好工具。

材料与方法

本研究共纳入44例早产新生儿，体重均小于2500g。从每个新生儿采集血样和鼻咽灌洗液。通过酚-氯仿提取技术获得白细胞DNA。采用PCR终点法扩增ompA基因来检测沙眼衣原体。还进行了细胞培养试验以及通过微量免疫荧光测定法检测抗沙眼衣原体的IgM抗体。

结果

20例新生儿PCR检测呈阳性（45.5%），该检测与肺炎的存在显著相关（相对危险度=2.28；95%置信区间：1.01至5.17；P=0.035）。鼻咽灌洗液的细胞培养仅7份样本呈阳性，未观察到与任何临床或实验室数据有显著关联。与PCR阳性相关的抗沙眼衣原体IgM抗体滴度为1:32（相对危险度=2.74；95%置信区间：1.21至6.23；P=0.008），然而该滴度与肺炎的存在无关。