Zhou Weidong, Zhang Yulei, Wen Yi, Ji Wei, Zhou Yang, Ji Yachan, Liu Xiaoling, Wang Weimin, Asim Muhammad, Liang Xufang, Ai Taoshan, Lin Li
Wuhan Fishery Research Institute, 430207 Wuhan, Hubei, China.
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
Fish Shellfish Immunol. 2015 Mar;43(1):111-9. doi: 10.1016/j.fsi.2014.12.006. Epub 2014 Dec 19.
Flavobacterium columnare (FC) is the causative pathogen of columnaris which has caused great economic loss in fish culture worldwide, including in Mandarin fish (Siniperca chuatsi) culture. In the present study, the transcriptomic profiles of the head kidneys from FC-infected and non-infected Mandarin fish were obtained using HiSeq™ 2000 (Illumina). Totally 31,168 unigenes with high quality were obtained. Genes involved in protein folding, metabolism and energy, immune responses, oxidoreductase activity, cell growth and death were identified as enriched classes. 1019 differently expressed genes between the two groups were identified, including 603 up-regulated and 416 down-regulated genes. 27 differently expressed immune related genes were scrutinized, including 17 up-regulated and 10 down-regulated genes. Six of the differently expressed genes were further validated by qRT-PCR. The roles of the immune related genes were discussed. Identification of the host genes in response to FC infection will shed a new light on the prevention of columnaris.
柱状黄杆菌（FC）是柱状病的致病病原体，该病在全球鱼类养殖中造成了巨大的经济损失，包括在鳜鱼（Siniperca chuatsi）养殖中。在本研究中，使用HiSeq™ 2000（Illumina）获得了感染FC和未感染FC的鳜鱼头部肾脏的转录组图谱。共获得31168个高质量的单基因。参与蛋白质折叠、代谢与能量、免疫反应、氧化还原酶活性、细胞生长和死亡的基因被确定为富集类别。鉴定出两组之间有1019个差异表达基因，包括603个上调基因和416个下调基因。对27个差异表达的免疫相关基因进行了详细研究，包括17个上调基因和10个下调基因。其中6个差异表达基因通过qRT-PCR进一步验证。讨论了免疫相关基因的作用。鉴定宿主对FC感染的反应基因将为柱状病的预防提供新的思路。
