Zhao Lijuan, Tu Jiagang, Zhang Yulei, Wang Jinfu, Yang Ling, Wang Weimin, Wu Zaohe, Meng Qinglei, Lin Li
Shandong Freshwater Fisheries Research Institute, Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Jinan, Shandong, 250013, China; Department of Aquatic Animal Medicine, College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Department of Aquatic Animal Medicine, College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Fish Shellfish Immunol. 2016 Oct;57:413-418. doi: 10.1016/j.fsi.2016.09.001. Epub 2016 Sep 4.
Flavobacterium columnare (FC) has caused worldwide fish columnaris disease with high mortality and great economic losses in cultured fish, including Topmouth culter (Culter alburnus). However, the knowledge about the host factors involved in FC infection is little known. In this study, the transcriptomic profiles of the head kidney from Topmouth culter with or without FC infection were obtained using HiSeq™ 2500 (Illumina). Totally 79,641 unigenes with high quality were obtained. Among them, 4037 differently expressed genes, including 1217 up-regulated and 2820 down-regulated genes, were identified and enriched using databases of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The differently expressed genes were mainly associated with pathways such as immune response, carbohydrate metabolism, amino acid metabolism, and lipid metabolism. Since phagocytosis is a central mechanism of innate immune response by host cells to defense against infectious agents, genes related to the phagosome pathway were scrutinized and 9 differently expressed phagosome-related genes were identified including 3 up-regulated and 6 down-regulated genes. Five of them were further validated by quantitative real-time polymerase chain reaction (qRT-PCR). This transcriptomic analysis of host genes in response to FC infection provides data towards understanding the infection mechanisms and will shed a new light on the prevention of columnaris.
柱状黄杆菌（FC）已在全球范围内引发鱼类柱状病，给包括翘嘴鲌（Culter alburnus）在内的养殖鱼类带来高死亡率和巨大经济损失。然而，关于参与FC感染的宿主因子的了解却很少。在本研究中，利用HiSeq™ 2500（Illumina）获得了感染或未感染FC的翘嘴鲌头肾的转录组图谱。共获得了79,641个高质量的单基因。其中，利用基因本体论（GO）和京都基因与基因组百科全书（KEGG）数据库鉴定并富集了4037个差异表达基因，包括1217个上调基因和2820个下调基因。差异表达基因主要与免疫反应、碳水化合物代谢、氨基酸代谢和脂质代谢等途径相关。由于吞噬作用是宿主细胞抵御感染因子的固有免疫反应的核心机制，因此对与吞噬体途径相关的基因进行了仔细研究，鉴定出9个差异表达的吞噬体相关基因，包括3个上调基因和6个下调基因。其中5个基因通过定量实时聚合酶链反应（qRT-PCR）进一步验证。这种对宿主基因响应FC感染的转录组分析为理解感染机制提供了数据，并将为柱状病的预防提供新的思路。
