Sokołowska-Wojdyło Małgorzata, Gleń Jolanta, Zabłotna Monika, Rębała Krzysztof, Trzeciak Magdalena, Sikorska Monika, Ruckemann-Dziurdzińska Katarzyna, Nedoszytko Boguslaw, Florek Aleksandra, Nowicki Roman
Department of Dermatology, Venereology and Allergology, Medical University of Gdańsk, Gdańsk, Poland.
Int J Dermatol. 2015 Jan;54(1):62-7. doi: 10.1111/ijd.12666.
Severe pruritus is one of the cardinal symptoms of atopic dermatitis (AD). Recently, the interleukin (IL)-31 cytokine has been implicated in the induction and maintenance of severe pruritus and chronic skin inflammation in several pruritic skin diseases, including AD.
We aimed to investigate the association of the IL-31 gene haplotypes with pruritus and severity of AD, as well as their correlation to the serum IL-31 levels.
A total of 127 patients with AD and 96 healthy controls were analyzed for polymorphic variants of the IL-31 gene using an amplification refractory mutation system-polymerase chain reaction method. IL-31 haplotype frequencies were estimated with the use of tagging single nucleotide polymorphisms, expectation-maximization, and Excoffier-Laval-Balding algorithms. Serum IL-31 levels were measured using a standard enzyme-linked immunosorbent assay kit.
The frequency of AAG, AGA, AGG, and GAA haplotypes of the IL-31 gene was higher in patients with AD than in controls. The mean IL-31 levels in serum were lower in controls than in the patients (P < 0.00001) and were higher in those with severe vs. mild AD (P = 0.008). No correlation was found between IL-31 and the severity of pruritus. The haplotype AAA was associated with a high IL-31 serum level (P = 0.008) and with severe AD (high SCORing Atopic Dermatitis index) (P = 0.013). The haplotype GAA was associated with a severe form of pruritus (P = 0.016) and the haplotype GGG with the mild one (P = 0.07).
The results of this study suggest that the severity of AD in a Polish population is associated with some specific haplotypes of the IL-31 gene, which can indicate their prognostic role also renews the questions concerning the role of IL-31 in pruritus in AD.
严重瘙痒是特应性皮炎（AD）的主要症状之一。最近，白细胞介素（IL）-31细胞因子在包括AD在内的几种瘙痒性皮肤病的严重瘙痒和慢性皮肤炎症的诱导和维持中发挥作用。
我们旨在研究IL-31基因单倍型与AD瘙痒及严重程度的关联，以及它们与血清IL-31水平的相关性。
使用扩增阻滞突变系统-聚合酶链反应方法对127例AD患者和96例健康对照者的IL-31基因多态性变异进行分析。使用标签单核苷酸多态性、期望最大化和Excoffier-Laval-Balding算法估计IL-31单倍型频率。使用标准酶联免疫吸附测定试剂盒测量血清IL-31水平。
AD患者中IL-31基因的AAG、AGA、AGG和GAA单倍型频率高于对照组。对照组血清中IL-31的平均水平低于患者（P<0.00001），重度AD患者高于轻度AD患者（P=0.008）。未发现IL-31与瘙痒严重程度之间存在相关性。单倍型AAA与高血清IL-31水平相关（P=0.008），与重度AD（高特应性皮炎评分指数）相关（P=0.013）。单倍型GAA与严重瘙痒形式相关（P=0.016），单倍型GGG与轻度瘙痒相关（P=0.07）。
本研究结果表明，波兰人群中AD的严重程度与IL-31基因的某些特定单倍型相关，这表明它们的预后作用，也再次引发了关于IL-31在AD瘙痒中作用的问题。
