Adal Ayelign M, Demissie Zerihun A, Mahmoud Soheil S
Department of Biology, University of British Columbia, 1177 Research Rd, Kelowna, BC, V1V 1V7, Canada.
Planta. 2015 Apr;241(4):987-1004. doi: 10.1007/s00425-014-2226-8. Epub 2014 Dec 23.
We identified and characterized EST-SSRs with strong discrimination power against Lavandula angustifolia and Lavandula x intermedia . The markers also showed considerable cross-species transferability rate into six related Lavandula species. Lavenders (Lavandula) are important economical crops grown around the globe for essential oil production. In an attempt to develop genetic markers for these plants, we analyzed over 13,000 unigenes developed from L. angustifolia and L. x intermedia EST databases, and identified 3,459 simple sequence repeats (SSR), which were dominated by trinucleotides (41.2 %) and dinucleotides (31.45 %). Approximately, 19 % of the unigenes contained at least one SSR marker, over 60 % of which were localized in the UTRs. Only 252 EST-SSRs were 18 bp or longer from which 31 loci were validated, and 24 amplified discrete fragments with 85 % polymorphism in L. x intermedia and L. angustifolia. The average number of alleles in L. x intermedia and L. angustifolia were 3.42 and 3.71 per marker with average PIC values of 0.47 and 0.52, respectively. These values suggest a moderate to strong level of informativeness for the markers, with some loci producing unique fingerprints. The cross-species transferability rate of the markers ranges 50-100 % across eight species. The utility of these markers was assessed in eight Lavandula species and 15 L. angustifolia and L. x intermedia cultivars, and the dendrogram deduced from their similarity indexes successfully delineated the species into their respective sections and the cultivars into their respective species. These markers have potential for application in fingerprinting, diversity studies and marker-assisted breeding of Lavandula.
我们鉴定并表征了对狭叶薰衣草和杂种薰衣草具有强大鉴别能力的EST-SSR。这些标记在六个相关薰衣草物种中也显示出相当高的跨物种转移率。薰衣草是全球种植的重要经济作物，用于生产精油。为了开发这些植物的遗传标记，我们分析了从狭叶薰衣草和杂种薰衣草EST数据库中开发的13000多个单基因，鉴定出3459个简单序列重复（SSR），其中以三核苷酸（41.2%）和二核苷酸（31.45%）为主。大约19%的单基因至少包含一个SSR标记，其中60%以上位于非翻译区。只有252个EST-SSR长度为18 bp或更长，其中31个位点得到验证，24个扩增出离散片段，在杂种薰衣草和狭叶薰衣草中具有85%的多态性。杂种薰衣草和狭叶薰衣草中每个标记的平均等位基因数分别为3.42和3.71，平均PIC值分别为0.47和0.52。这些值表明这些标记具有中等至较强的信息性水平，一些位点产生独特的指纹。这些标记在八个物种中的跨物种转移率为50-100%。在八个薰衣草物种以及15个狭叶薰衣草和杂种薰衣草品种中评估了这些标记的实用性，根据相似性指数推导的聚类图成功地将物种划分为各自的组，将品种划分为各自的物种。这些标记在薰衣草的指纹识别、多样性研究和标记辅助育种中具有应用潜力。
