Department of Biology and Geology, University of Almeria, 04120, Almeria, Spain.
Department of Engineering, University of Almeria, 04120, Almeria, Spain.
Planta. 2018 Mar;247(3):543-557. doi: 10.1007/s00425-017-2829-y. Epub 2017 Dec 14.
A deep bibliometric analysis has been carried out, obtaining valuable parameters that facilitate the understanding around the research in plant using molecular markers. The evolution of the improvement in the field of agronomy is fundamental for its adaptation to the new exigencies that the current world context raises. In addition, within these improvements, this article focuses on those related to the biotechnology sector. More specifically, the use of DNA markers that allow the researcher to know the set of genes associated with a particular quantitative trait or QTL. The use of molecular markers is widely extended, including: restriction fragment length polymorphism, random-amplified polymorphic DNA, amplified fragment length polymorphism, microsatellites, and single-nucleotide polymorphisms. In addition to classical methodology, new approaches based on the next generation sequencing are proving to be fundamental. In this article, a historical review of the molecular markers traditionally used in plants, since its birth and how the new molecular tools facilitate the work of plant breeders is carried out. The evolution of the most studied cultures from the point of view of molecular markers is also reviewed and other parameters whose prior knowledge can facilitate the approach of researchers to this field of research are analyzed. The bibliometric analysis of molecular markers in plants shows that top five countries in this research are: US, China, India, France, and Germany, and from 2013, this research is led by China. On the other hand, the basic research using Arabidopsis is deeper in France and Germany, while other countries focused its efforts in their main crops as the US for wheat or maize, while China and India for wheat and rice.
进行了深入的文献计量分析,获得了有价值的参数,有助于理解使用分子标记进行植物研究。农学领域的改进演变对于适应当前世界背景提出的新要求至关重要。此外,在这些改进中,本文侧重于与生物技术领域相关的改进。更具体地说,使用 DNA 标记可以让研究人员了解与特定数量性状或 QTL 相关的基因集。分子标记的使用得到了广泛的扩展,包括:限制性片段长度多态性、随机扩增多态性 DNA、扩增片段长度多态性、微卫星和单核苷酸多态性。除了经典方法外,基于下一代测序的新方法也被证明是至关重要的。本文对植物中传统使用的分子标记进行了历史回顾,从其诞生到新的分子工具如何促进植物育种者的工作,对其进行了回顾。还回顾了从分子标记角度研究最多的文化的演变,并分析了其他可以帮助研究人员了解该研究领域的先验知识参数。对植物分子标记的文献计量分析表明,在这项研究中排名前五的国家是:美国、中国、印度、法国和德国,自 2013 年以来,中国主导了这项研究。另一方面,法国和德国在使用拟南芥进行基础研究方面更为深入,而其他国家则将重点放在其主要作物上,如美国的小麦或玉米,而中国和印度则侧重于小麦和水稻。