Soliman Hala M, Wagih Heba M, Attia Ghalia M, Algaidi Sami A
Department of Anatomy, Faculty of Medicine, Taibah University, Al Madina Al Monawarrah, Kingdom of Saudi Arabia; Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Folia Histochem Cytobiol. 2014;52(4):335-49. doi: 10.5603/FHC.a2014.0038. Epub 2014 Dec 23.
Sexual dysfunction and infertility are symptoms which have been rarely studied in patients treated with antischizophrenic drugs, aripiprazole and olanzapine, for long period. This work aimed to investigate the effects of aripiprazole and olanzapine on the structure of seminiferous tubules of rats at both light microscopic and ultrastructural levels.
Sixty adult male rats were divided into 3 groups (n = 20): control group (Group I) and two experimental ones (II and III). Rats in Group II received 2 mg/kg/day aripiprazole while rats in Group III received 0.5 mg/kg/day olanzapine for 14 weeks. Thereafter, testis were removed and processed for both light and electron microscopic study. Qualitative morphological analyses and histomorphometric measurements of seminiferous tubules were performed.
Rats in Group II showed reduction of testicular weight, seminiferous tubules' diameter, epithelial height, spermatogenic count, spermatogenic index and spermatogenic score whereas Sertoli cells count was increased. Olanzapine-treated rats also showed epithelial desquamation, separation and apoptotic changes of germ cells. Sertoli cells showed vacuolization, dilatation of smooth endoplasmic reticulum and accumulation of lipid droplets. Abnormality in the shape and structure of late spermatids and presence of giant cells were also demonstrated. Aripiprazole induced less adverse histological changes in rat testis than olanzapine.
Olanzapine followed by aripiprazole had adverse histological effects on the structure of the seminiferous tubules, which may affect spermatogenesis.
性功能障碍和不育是长期使用抗精神分裂症药物阿立哌唑和奥氮平治疗的患者中很少被研究的症状。这项工作旨在从光镜和超微结构水平研究阿立哌唑和奥氮平对大鼠生精小管结构的影响。
60只成年雄性大鼠分为3组（n = 20）：对照组（I组）和两个实验组（II组和III组）。II组大鼠接受2 mg/kg/天的阿立哌唑，而III组大鼠接受0.5 mg/kg/天的奥氮平，持续14周。此后，取出睾丸并进行光镜和电镜研究。对生精小管进行定性形态学分析和组织形态计量学测量。
II组大鼠睾丸重量、生精小管直径、上皮高度、生精细胞计数、生精指数和生精评分降低，而支持细胞计数增加。奥氮平治疗的大鼠还出现生殖细胞上皮脱落、分离和凋亡变化。支持细胞出现空泡化、滑面内质网扩张和脂滴积累。还证实了晚期精子细胞的形状和结构异常以及巨细胞的存在。与奥氮平相比，阿立哌唑对大鼠睾丸的不良组织学变化较小。
奥氮平继以阿立哌唑对生精小管结构有不良组织学影响，这可能会影响精子发生。
