Abdellatief R B, Elgamal D A, Mohamed E E M
Department of Pharmacology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Department of Histology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Andrologia. 2015 Aug;47(6):674-9. doi: 10.1111/and.12316. Epub 2014 Sep 16.
In a prospective experimental study, the effects of chronic tramadol administration on gonadotrophic and sex hormones, histopathological and morphometrical alterations in rat testicular tissue were investigated in a laboratory setting. Tramadol was given alone to adult male albino rats. Gonadotrophic and serum sex hormone levels were measured and testicular pathological and morphometric changes were observed in treated vs.
After 30 days of treatment, tramadol induced a decrease in LH, FSH and testosterone serum levels. Histologically, degenerative changes in the seminiferous tubules were observed. They showed shrinkage, separation of tubular basement membrane, disorganisation and vacuolisation of spermatogenic layers. Morphometric analysis revealed significant decrease in the mean values of the tubular diameter and epithelial height. Ultrastructural abnormalities were detected in all cells of spermatogenic lineage in addition to the appearance of apoptotic cells. Sertoli cell vacuolation, huge lipid droplets and disrupted Sertoli cell junctions were observed. Leydig cells showed euchromatic nuclei and dilated smooth endoplasmic reticulum. In view of these findings, it is concluded that tramadol induces alterations in sex hormonal levels in conjunction with disruption of the normal histological structure of rat testis. This might lead to the risk of male infertility. Therefore, tramadol should be used with caution with appropriate dose monitoring.
在一项前瞻性实验研究中，在实验室环境下研究了长期给予曲马多对大鼠睾丸组织中促性腺激素和性激素、组织病理学及形态学改变的影响。单独给成年雄性白化大鼠给予曲马多。测量了促性腺激素和血清性激素水平，并观察了治疗组与对照组睾丸的病理和形态学变化。
治疗30天后，曲马多导致血清促黄体生成素（LH）、促卵泡生成素（FSH）和睾酮水平降低。组织学上，观察到生精小管出现退行性变化。它们表现为萎缩、管状基底膜分离、生精层紊乱和空泡化。形态学分析显示管腔直径和上皮高度的平均值显著降低。除了出现凋亡细胞外，在生精谱系的所有细胞中都检测到超微结构异常。观察到支持细胞空泡化、巨大脂滴和支持细胞连接破坏。睾丸间质细胞显示核染色质浅染和滑面内质网扩张。鉴于这些发现，得出结论，曲马多会导致性激素水平改变，并伴有大鼠睾丸正常组织结构的破坏。这可能会导致男性不育的风险。因此，使用曲马多时应谨慎并进行适当的剂量监测。
