Flores-Garcia Aurelio, Torres-Bugarin Olivia, Salvador Velarde-Félix Jesús, Rangel-Villalobos Héctor, Zepeda-Carrillo Eloy Alfonso, Rodríguez-Trejo Amelia, Aguíar-García Pedro, Nersesyan Armen
Unidad Académica de Medicina, Universidad Autónoma de Nayarit; Tepic, Nayarit, México;
J BUON. 2014 Oct-Dec;19(4):895-9.
Breast cancer (BC) is the most frequently diagnosed form of cancer and the leading cause of cancer-related deaths among females in the world. RESULTS of several studies showed that the genome of primary cancer patients (naive for any treatment) is unstable. The purpose of the present study was to evaluate the genomic instability in BC patients by means of buccal cells micronucleus (MN) cytome assay Methods: The frequencies of nuclear anomalies including MN, binucleates (BN), broken eggs (BE), condensed chromatin (CC), karyorrhexis (KR) and karyolysis (KL) were evaluated in exfoliated buccal mucosa cells of Mexican women with primary BC and healthy women. Buccal cells were collected from 21 BC patients (9 with stage I and 12 with stage II) and from 20 healthy females used as control group.
The results of the evaluation of cells showed that the frequencies of MN, BN, BE, KR and KL were significantly increased in the pooled group of BC patients compared with the control group. However, no one parameter of buccal MN-cytome assay in patients with stage I BC was significant compared with controls and BC patients with stage II.
Application of the buccal MN-cytome assay for the study of genomic instability in primary BC patients showed that both genotoxic and cytotoxic effects can be evaluated in such patients.
乳腺癌（BC）是世界上女性中最常被诊断出的癌症形式，也是癌症相关死亡的主要原因。多项研究结果表明，原发性癌症患者（未接受过任何治疗）的基因组不稳定。本研究的目的是通过颊细胞微核（MN）细胞分析法评估乳腺癌患者的基因组不稳定性。方法：在患有原发性乳腺癌的墨西哥女性和健康女性的脱落颊黏膜细胞中，评估包括微核（MN）、双核（BN）、碎核（BE）、凝聚染色质（CC）、核碎裂（KR）和核溶解（KL）在内的核异常频率。从21例乳腺癌患者（9例I期和12例II期）以及20名作为对照组的健康女性中采集颊细胞。
细胞评估结果显示，与对照组相比，乳腺癌患者合并组中微核、双核、碎核、核碎裂和核溶解的频率显著增加。然而，与对照组和II期乳腺癌患者相比，I期乳腺癌患者颊部微核细胞分析法的任何一个参数均无显著性差异。
颊部微核细胞分析法在原发性乳腺癌患者基因组不稳定性研究中的应用表明，此类患者的遗传毒性和细胞毒性作用均可得到评估。
