Bai Xiangyang, Li Linlin, Liu Huiyu, Tan Longfei, Liu Tianlong, Meng Xianwei
Laboratory of Controllable Preparation and Application of Nanomaterials, Center for Micro/nanomaterials and Technology, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
ACS Appl Mater Interfaces. 2015 Jan 21;7(2):1308-17. doi: 10.1021/am507532p. Epub 2015 Jan 9.
Zinc oxide nanoparticles (ZnONPs) have been widely studied as the bacteriostatic reagents. However, synthesis of small ZnO nanoparticles with good monodispersion and stability in aqueous solution is still a challenge. Anti-infection research of ZnONPs used as antibacterial agent in vivo is rare. In this paper, a novel, sustainable, and simple method to synthesize ZnO nanoparticles with good monodispersion in aqueous low-temperature conditions and with a small molecule agent is reported. Inhibition zone test and the minimum inhibitory concentration test were performed to examine the antibacterial activity of ZnONPs against bacteria Staphylococcus aureus and Escherichia coli in vitro. For further application in vivo, low cytotoxicity and low acute toxicity in mice of ZnO were demonstrated. Finally, 4 nm ZnONPs combined with poly(vinyl alcohol) gel was used as antibacterial agent in rodent elytritis model, and significant anti-infection effect was proven. In one word, the present research would shed new light on the designing of antibacterial materials like ZnO with promising application in disinfection.
氧化锌纳米颗粒（ZnONPs）作为抑菌剂已得到广泛研究。然而，在水溶液中合成具有良好单分散性和稳定性的小尺寸ZnO纳米颗粒仍然是一项挑战。ZnONPs作为抗菌剂在体内的抗感染研究很少。本文报道了一种新颖、可持续且简单的方法，即在低温水溶液条件下用小分子试剂合成具有良好单分散性的ZnO纳米颗粒。进行抑菌圈试验和最低抑菌浓度试验，以检测ZnONPs对金黄色葡萄球菌和大肠杆菌的体外抗菌活性。为了进一步在体内应用，证明了ZnO对小鼠具有低细胞毒性和低急性毒性。最后，将4 nm的ZnONPs与聚乙烯醇凝胶结合用作啮齿动物鞘炎模型中的抗菌剂，并证明了显著的抗感染效果。总之，本研究将为设计如ZnO这样在消毒方面具有广阔应用前景的抗菌材料提供新的思路。
