São Paulo State University (UNESP), School of Pharmaceutical Sciences, Department of Drugs and Medicines, Highway Araraquara-Jaú, Araraquara, SP, Brazil.
São Paulo State University (UNESP), School of Pharmaceutical Sciences, Department of Drugs and Medicines, Highway Araraquara-Jaú, Araraquara, SP, Brazil; Department of Biological and Health Sciences, Universidade de Araraquara, UNIARA, Araraquara, SP, Brazil.
Colloids Surf B Biointerfaces. 2019 May 1;177:440-447. doi: 10.1016/j.colsurfb.2019.02.013. Epub 2019 Feb 11.
In the current study, the size and surface of ZnO nanoparticle (ZnO NP) suspensions and powders were finely controlled to evaluate their influence on the ZnO antibacterial activity against Staphylococcus aureus and Escherichia coli. The ZnO NP were prepared by the sol-gel method with different reaction times for NP size control and followed by the addition of (3-glycidyloxypropyl) trimethoxysilane (GPTMS) as a surface modifier. The ZnO NP were characterized by different techniques and the antibacterial activity was assessed through the minimum inhibitory concentration assay (MIC), minimum bactericidal concentration assay (MBC) and scanning electron microscopy (SEM). The ZnO NP exhibited significant antibacterial activity against Staphylococcus aureus. The NP size highly influenced the antibacterial activity, which increased with decreasing particle size. The small ZnO NP presented bactericidal activity whereas the largest showed bacteriostatic activity. The use of GPTMS, in general, led to increase of MIC and MBC. The formation of holes in the cell wall of Staphylococcus aureus was evidenced by SEM after contact between the bacteria and ZnO NP. The cytotoxicity assay showed that ZnO NP did not cause a loss of cell viability in the human keratinocyte cell line (HaCat) at the maximum concentration assessed. Thus, this study indicated that 5 nm ZnO NP modified by GPTMS has great potential for use as an inorganic antibacterial material.
在本研究中，精细控制了氧化锌纳米粒子（ZnO NP）悬浮液和粉末的尺寸和表面，以评估它们对 ZnO 抗菌活性（针对金黄色葡萄球菌和大肠杆菌）的影响。通过溶胶-凝胶法制备 ZnO NP，通过不同的反应时间控制 NP 的尺寸，并随后添加（3-缩水甘油氧基丙基）三甲氧基硅烷（GPTMS）作为表面改性剂。通过不同的技术对 ZnO NP 进行了表征，并通过最低抑菌浓度测定（MIC）、最低杀菌浓度测定（MBC）和扫描电子显微镜（SEM）评估了抗菌活性。ZnO NP 对金黄色葡萄球菌表现出显著的抗菌活性。NP 尺寸对抗菌活性有很大影响，随着粒径的减小而增加。小的 ZnO NP 具有杀菌活性，而最大的 ZnO NP 具有抑菌活性。一般来说，GPTMS 的使用导致 MIC 和 MBC 的增加。SEM 显示，在细菌与 ZnO NP 接触后，金黄色葡萄球菌细胞壁形成了孔。细胞毒性试验表明，在评估的最大浓度下，ZnO NP 不会导致人角质形成细胞系（HaCat）丧失细胞活力。因此，本研究表明，经 GPTMS 修饰的 5nm ZnO NP 具有作为无机抗菌材料的巨大潜力。
