Varsani Arvind, Porzig Elizabeth L, Jennings Scott, Kraberger Simona, Farkas Kata, Julian Laurel, Massaro Melanie, Ballard Grant, Ainley David G
Electron Microscope Unit, Division of Medical Biochemistry, Department of Clinical Laboratory Sciences, University of Cape Town, Observatory, 7700, South Africa.
Department of Plant Pathology and Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.
J Gen Virol. 2015 Apr;96(Pt 4):851-857. doi: 10.1099/vir.0.000038. Epub 2014 Dec 23.
Little is known about viruses associated with Antarctic animals, although they are probably widespread. We recovered a novel polyomavirus from Adélie penguin (Pygoscelis adeliae) faecal matter sampled in a subcolony at Cape Royds, Ross Island, Antarctica. The 4988 nt Adélie penguin polyomavirus (AdPyV) has a typical polyomavirus genome organization with three ORFs that encoded capsid proteins on the one strand and two non-structural protein-coding ORFs on the complementary strand. The genome of AdPyV shared ~60 % pairwise identity with all avipolyomaviruses. Maximum-likelihood phylogenetic analysis of the large T-antigen (T-Ag) amino acid sequences showed that the T-Ag of AdPyV clustered with those of avipolyomaviruses, sharing between 48 and 52 % identities. Only three viruses associated with Adélie penguins have been identified at a genomic level, avian influenza virus subtype H11N2 from the Antarctic Peninsula and, respectively, Pygoscelis adeliae papillomavirus and AdPyV from capes Crozier and Royds on Ross Island.
尽管与南极动物相关的病毒可能广泛存在，但人们对它们知之甚少。我们从南极罗斯岛罗伊兹角一个亚群落采集的阿德利企鹅（Pygoscelis adeliae）粪便中分离出一种新型多瘤病毒。这种4988个核苷酸的阿德利企鹅多瘤病毒（AdPyV）具有典型的多瘤病毒基因组结构，一条链上有三个编码衣壳蛋白的开放阅读框（ORF），互补链上有两个非结构蛋白编码ORF。AdPyV的基因组与所有禽多瘤病毒的成对同一性约为60%。对大T抗原（T-Ag）氨基酸序列进行的最大似然系统发育分析表明，AdPyV的T-Ag与禽多瘤病毒的T-Ag聚类在一起，同一性在48%至52%之间。在基因组水平上，仅鉴定出三种与阿德利企鹅相关的病毒，分别是来自南极半岛的H11N2亚型禽流感病毒，以及来自罗斯岛克罗泽角和罗伊兹角的阿德利企鹅乳头瘤病毒和AdPyV。
