Department of Nursing College, Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
Department of Orthopedics, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
Neural Regen Res. 2012 Oct 5;7(28):2182-7. doi: 10.3969/j.issn.1673-5374.2012.028.003.
Muscle-derived stem cells were isolated from the skeletal muscle of Sprague-Dawley neonatal rats aged 3 days old. Cells at passage 5 were incubated in Dulbecco's modified Eagle's medium supplemented with 10% (v/v) fetal bovine serum, 20 μg/L nerve growth factor, 20 μg/L basic fibroblast growth factor and 1% (v/v) penicillin for 6 days. Cells presented with long processes, similar to nerve cells. Connections were formed between cell processes. Immunocytochemical staining with neuron specific enolase verified that cells differentiated into neuron-like cells. Immunofluorescence cytochemistry and western blot results revealed that the expression of protein hairy enhancer of split-1 was significantly reduced. These results indicate that low expression of protein hairy enhancer of split-1 participates in the differentiation of muscle-derived stem cells into neuron-like cells.
肌肉来源的干细胞从 3 天大的 Sprague-Dawley 新生大鼠的骨骼肌中分离得到。传代 5 代的细胞在添加 10%（v/v）胎牛血清、20 μg/L 神经生长因子、20 μg/L 碱性成纤维细胞生长因子和 1%（v/v）青霉素的 Dulbecco 改良 Eagle 培养基中孵育 6 天。细胞呈现出长突起，类似于神经细胞。细胞突起之间形成连接。神经元特异性烯醇化酶的免疫细胞化学染色证实细胞分化为类神经元细胞。免疫荧光细胞化学和 Western blot 结果显示，蛋白 hairy enhancer of split-1 的表达明显降低。这些结果表明，蛋白 hairy enhancer of split-1 的低表达参与了肌肉来源的干细胞向类神经元细胞的分化。
