Division of Neonatology, Affiliated Children's Hospital of Soochow University, Suzhou 215003, Jiangsu Province, China.
Department of General Pediatrics, Yixing People's Hospital, Yixing 214200, Jiangsu Province, China.
Neural Regen Res. 2012 Oct 5;7(28):2221-6. doi: 10.3969/j.issn.1673-5374.2012.028.008.
Clock genes are involved in circadian rhythm regulation, and surviving newborns with hypoxic-ischemic encephalopathy may present with sleep-wake cycle reversal. This study aimed to determine the expression of the clock genes Clock and Bmal1, in the pineal gland of rats with hypoxic-ischemic brain damage. Results showed that levels of Clock mRNA were not significantly changed within 48 hours after cerebral hypoxia and ischemia. Expression levels of CLOCK and BMAL1 protein were significantly higher after 48 hours. The levels of Bmal1 mRNA reached a peak at 36 hours, but were significantly reduced at 48 hours. Experimental findings indicate that Clock and Bmal1 genes were indeed expressed in the pineal glands of neonatal rats. At the initial stage (within 36 hours) of hypoxic-ischemic brain damage, only slight changes in the expression levels of these two genes were detected, followed by significant changes at 36-48 hours. These changes may be associated with circadian rhythm disorder induced by hypoxic-ischemic brain damage.
时钟基因参与昼夜节律调节，患有缺氧缺血性脑病的存活新生儿可能会出现睡眠-觉醒周期逆转。本研究旨在确定缺氧缺血性脑损伤大鼠松果体中时钟基因 Clock 和 Bmal1 的表达。结果显示，脑缺氧缺血后 48 小时内 Clock mRNA 水平无明显变化。48 小时后，CLOCK 和 BMAL1 蛋白表达水平显著升高。Bmal1 mRNA 水平在 36 小时达到峰值，但在 48 小时显著降低。实验结果表明，Clock 和 Bmal1 基因确实在新生大鼠的松果体中表达。在缺氧缺血性脑损伤的初始阶段（36 小时内），仅检测到这两个基因表达水平的轻微变化，随后在 36-48 小时内发生显著变化。这些变化可能与缺氧缺血性脑损伤引起的昼夜节律紊乱有关。
