通过内质网相关降解介导的Env耗竭降低HIV-1感染性。
Reduction of HIV-1 infectivity through endoplasmic reticulum-associated degradation-mediated Env depletion.
作者信息
Casini Antonio, Olivieri Michele, Vecchi Lara, Burrone Oscar R, Cereseto Anna
机构信息
Centre for Integrative Biology (CIBIO), University of Trento, Trento, Italy
Centre for Integrative Biology (CIBIO), University of Trento, Trento, Italy.
出版信息
J Virol. 2015 Mar;89(5):2966-71. doi: 10.1128/JVI.02634-14. Epub 2014 Dec 24.
Abstract
During the HIV-1 replicative cycle, the gp160 envelope is processed in the secretory pathway to mature into the gp41 and gp120 subunits. Misfolded proteins located within the endoplasmic reticulum (ER) are proteasomally degraded through the ER-associated degradation (ERAD) pathway, a quality control system operating in this compartment. Here, we exploited the ERAD pathway to induce the degradation of gp160 during viral production, thus leading to the release of gp120-depleted viral particles.
摘要
在HIV-1复制周期中，gp160包膜蛋白在分泌途径中被加工成熟为gp41和gp120亚基。位于内质网（ER）内的错误折叠蛋白通过内质网相关降解（ERAD）途径被蛋白酶体降解，这是该细胞器中运行的一种质量控制系统。在此，我们利用ERAD途径在病毒产生过程中诱导gp160的降解，从而导致释放缺乏gp120的病毒颗粒。
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