Mirhadi Hosein, Azar Mohammad Reza, Abbaszadegan Abbas, Geramizadeh Bita, Torabi Shima, Rahsaz Marjan
Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Pathology, Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Dent Res J (Isfahan). 2014 Nov;11(6):645-8.
A strong antimicrobial synergism between chlorhexidine (CHX) and hydrogen peroxide (H2O2) has been reported, but there is not enough data on the cytotoxicity of this combination. The primary aim of this study was to evaluate the cytotoxicity of CHX-H2O2 combination in different concentrations and secondary aim is to assess the influence of H2O2 on cytotoxicity of CHX on cultured human periodontal ligament (PDL) fibroblasts.
The PDL cells were cultured from healthy human third molar teeth and were exposed to six prepared solutions (0.2% and 2% CHX separately and in combination with 1% and 3% H2O2). The MTT assay was applied to assess their effects on the viability of the PDL cells. Two-way analysis of variance approach and subgroup analysis was performed to evaluate the differences in mean cell viability values. A level of P < 0.05 was considered as statistically significant.
All tested solutions were toxic to PDL cells. There was a significant interaction effect between CHX and H2O2. The 2% CHX combined with 3% H2O2 was the most and 0.2% CHX was the least cytotoxic solutions. The 2% CHX was significantly more toxic than 0.2% CHX and H2O2 combinations. The cytotoxicity of 0.2% CHX and H2O2 combinations did not significantly rise by increasing the concentration of H2O2 from 1% to 3%.
H2O2 affected the cytotoxicity of CHX in a variable concentration-dependent manner. Based on the results of this study, it can be concluded that 2% CHX alone and in combination with either 1 or 3% H2O2 are significantly more toxic than 0.2% CHX alone and in combination with 1 and 3% H2O2. Therefore, to benefit from the synergistic antimicrobial effect between CHX and H2O2, with a minimal cytotoxicity, it is recommended to use 0.2% concentration of CHX combined with 3% H2O2.
已有报道称洗必泰（CHX）与过氧化氢（H2O2）之间存在强大的抗菌协同作用，但关于这种组合的细胞毒性数据不足。本研究的主要目的是评估不同浓度的CHX-H2O2组合的细胞毒性，次要目的是评估H2O2对CHX对培养的人牙周膜（PDL）成纤维细胞的细胞毒性的影响。
从健康人第三磨牙培养PDL细胞，并将其暴露于六种配制好的溶液中（分别为0.2%和2%的CHX，以及与1%和3%的H2O2组合）。采用MTT法评估它们对PDL细胞活力的影响。进行双向方差分析和亚组分析以评估平均细胞活力值的差异。P<0.05被认为具有统计学意义。
所有测试溶液对PDL细胞均有毒性。CHX和H2O2之间存在显著的交互作用。2%的CHX与3%的H2O2组合的细胞毒性最大，0.2%的CHX细胞毒性最小。2%的CHX比0.2%的CHX与H2O2组合的毒性显著更大。将H2O2浓度从1%提高到3%，0.2%的CHX与H2O2组合的细胞毒性没有显著增加。
H2O2以可变的浓度依赖性方式影响CHX的细胞毒性。根据本研究结果，可以得出结论，单独使用2%的CHX以及与1%或3%的H2O2组合的毒性显著高于单独使用0.2%的CHX以及与1%和3%的H2O2组合。因此，为了在最小细胞毒性的情况下受益于CHX和H2O2之间的协同抗菌作用，建议使用0.2%浓度的CHX与3%的H2O2组合。
